Phylogenomics identifies parents of naturally occurring tetraploid bananas
Journal
Botanical Studies
Journal Volume
65
Journal Issue
1
Pages
19
Date Issued
2024-07
Author(s)
Abstract
BackgroundTriploid bananas are almost sterile. However, we succeeded in harvesting seeds from two edible triploid banana individuals (Genotype: ABB) in our conservation repository where various wild diploid bananas were also grown. The resulting rare offspring survived to seedling stages. DNA content analyses reveal that they are tetraploid. Since bananas contain maternally inherited plastids and paternally inherited mitochondria, we sequenced and assembled plastomes and mitogenomes of these seedlings to trace their hybridization history.ResultsThe coding sequences of both organellar genomic scaffolds were extracted, aligned, and concatenated for constructing phylogenetic trees. Our results suggest that these tetraploid seedlings be derived from hybridization between edible triploid bananas and wild diploid Musa balbisiana (BB) individuals. We propose that generating female triploid gametes via apomeiosis may allow the triploid maternal bananas to produce viable seeds.ConclusionsOur study suggests a practical avenue towards expanding genetic recombination and increasing genetic diversity of banana breeding programs. Further cellular studies are needed to understand the fusion and developmental processes that lead to formation of hybrid embryos in banana reproduction, polyploidization, and evolution.
Subjects
banana
hybridization
Polyploidization
Apomeiosis
Triploid bridge
SDGs
Publisher
Springer Nature
Description
We greatly appreciate Taiwan Agricultural Research Institute for providing the plant materials. We thank the anonymous reviewers for their critical reading and valuable comments.DAS:All DNAseq reads used for the genome assembly are available in the NCBI SRA database under a BioProject number: PRJNA1066417. The assembled organellar genomes are deposited in the GenBank (Plastomes: LC792611-LC792623; mitochondrial scaffolds: LC794909-LC795246).
Type
journal article