以雙限制酶切位點標定法開發甘藍品種鑑別SNP套件
Other Title
Developing an SNP Assay Set for Brassica oleracea Using ddRADseq
Journal
農業部臺中區農業改良場研究彙報
Journal Issue
165
Start Page
35
End Page
48
ISSN
0255-5905
Date Issued
2024-12
Author(s)
Abstract
本研究以甘藍為對象，運用次世代定序技術中的ddRADseq進行基因體分析。通過對112個育種材料的定序與分析，成功識別745,196個變異位點，其中包括648,810個SNP位點。經嚴格的條件篩選，保留3,565個高品質SNP位點，並藉由這些SNP之基因型值，建構甘藍育種材料遺傳異質性及遺傳歧異性資料，為材料管理與育種提供豐富之參考訊息。由其中挑選8組可區分109個品系之SNP合成分析套組，於24個驗證材料中之可用性試驗結果顯示，本分析套組具高度的穩定性及可靠性。本研究不僅開發輔助甘藍育種之分子標誌套組，建立之甘藍ddRADseq試驗與分析流程可經濟且快速的獲得大量樣品的基因型值，可作為甘藍分子輔助育種及基因定位有利工具。
This study focuses on Brassica oleracea, a major vegetable in Taiwan, using the ddRADseq technique, a next-generation sequencing method, for genomic analysis. Through the sequencing and analysis of 112 breeding materials, a total of 745,196 variant loci were successfully identified, including 648,810 SNP loci. After rigorous filtering, 3,565 high-quality SNP loci were retained. By utilizing the genotypic values of these SNPs, genetic heterogeneity and diversity data for Brassica oleracea breeding materials were constructed, providing rich reference information for material management and breeding. Eight sets of synthetic analysis kits were selected from these SNPs, capable of distinguishing 109 different varieties, and their utility was confirmed in 24 validation materials. The analysis kits demonstrated high stability and reliability within breeding materials, serving as a powerful tool for breeding efforts. This study not only established a molecular marker kit to assist Brassica oleracea breeding but also introduced an economical and rapid ddRADseq experiment and analysis pipeline for obtaining genotypic values of a large number of samples. This pipeline can serve as a beneficial tool for molecular-assisted breeding and gene localization in Brassica oleracea.
Subjects
甘藍
單一核苷酸多型性
雙限制酶切位點標定法
cabbage
SNP
ddRAD
Publisher
農業部臺中區農業改良場
Type
other periodical