台灣地區根腐線蟲Pratylenchus coffeae (Nematoda: Pratylenchidae) 之鑑定

陳殿義; 倪蕙芳; 蔡東纂; 顏志恒; Chen, D. Y.; Ni, H. F.; Tsay, T. T.; Yen, J. H.; 陳殿義; 倪蕙芳; 蔡東纂; 顏志恒

台灣地區根腐線蟲Pratylenchus coffeae (Nematoda: Pratylenchidae) 之鑑定

Other Title

Identification of root-lesion nematode Pratylenchus coffeae (Nematoda: Pratylenchidae) occurred in Taiwan

Journal

植物病理學會刊

Journal Volume

20

Journal Issue

1-2

Pages

26-38

Date Issued

2011

Date

2011

Author(s)

陳殿義

倪蕙芳

蔡東纂

顏志恒

Chen, D. Y.

Ni, H. F.

Tsay, T. T.

Yen, J. H.

URI

https://scholars.tari.gov.tw/handle/123456789/4529

Abstract

自2007年10月起至2011年3月止，從台灣地區的甜橙、桶柑、文旦、檸檬、酪梨、葡萄的根部及山藥塊莖上，以改良式柏門氏漏斗分離法共計分離到8個根腐線蟲 (Pratylenchus spp) 種群。經由光學顯微鏡和SEM的形態特徵觀察，該8個根腐線蟲種群皆具有唇部低平，唇部上具有2個體環 (annules)，唇端上之唇盤 (labial disc) 和唇板 (labial plate) 融合而呈現為平滑面 (smooth face)；尾部尾端大多呈現截頭形 (truncate)，少數為鈍狀圓突形 (bluntly pointed)。另外由形態測量值 (morphometrics) 的比對分析，並輔以包含完整內轉錄區間 ITS-1和ITS-2和5.8S基因，以及部分18S和28S基因序列的核糖體DNA (ribosomal DNA, rDNA) 片段序列的相同度 (identity) 比較，因此將該8個根腐線蟲種群皆鑑定為P. coffeae (Zimmermann, 1898) Filipjev & Schuurmans Stekhoven, 1941。P. coffeae的rDNA片段代表性序列大小為1249 bp，其中ITS-1、ITS-2及5.8S的序列大小，依序分別為484 bp、311 bp及157 bp。 Eight populations of root-lesion nematode were isolated from the roots of sweet orange (Citrus sinensis), tankan (C. tankan), buntan (C. grandis f. buntan), lemon (C. limon), avocado (Persea americana), grape (Vitis vinifera) and the yam tubers using modified Baermann funnel method in Taiwan during October 2007 to March 2011. Morphological features observed via optical microscopy and SEM showed that the 8 nematode populations have low and flat lip region, with two annules; the labial disc and labial plate of the lip region are integrated into a smooth face and most of end-tail shapes are truncate, few are bluntly pointed. Based on the morphology, morphometrics and ribosomal DNA fragment sequences, including full 5.8S gene, ITS-1, ITS-2 and partial 18S and 28S gene, we have identified the 8 populations of root-lesion nematode as Pratylenchus coffeae (Zimmermann, 1898) Filipjev & Schuurmans Stekhoven, 1941. The length of representative rDNA fragment sequence of P. coffeae was 1249 bp, in which the lengths of ITS-1, ITS-2 and 5.8S gene were 484 bp, 311 bp and 157 bp, respectively.

Subjects

根腐線蟲

鑑定

形態測量值

核糖體 DNA

Pratylenchus coffeae

root-lesion nematode

identification

morphometrics

ribosomal DNA

Pratylenchus coffeae

Publisher

中華民國植物病理學會

Type

journal article

File(s)

Name

20-26-38(P. coffeae).pdf

Size

1.93 MB

Format

Adobe PDF

Checksum

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