The impact of transgenic papaya (TPY10-4) fruit supplementation on immune responses in ovalbumin-sensitised mice
Journal
Journal of the Science of Food and Agriculture
Journal Volume
91
Journal Issue
3
Pages
539-546
Date Issued
2011-02
Date
2011-02
Author(s)
Abstract
BACKGROUND: A transgenic papaya line (TPY10-4) that is resistant to both papaya ringspot virus (PRSV) and papaya leaf distortion mosaic virus (PLDMV) has been developed in Taiwan. This study investigated the immunomodulatory properties of transgenic TPY10-4 and its native (TCK) papaya fruits using an ovalbumin (OVA)-sensitised mouse model. Both green and ripe papaya fruits at low (0.2 g powder kg−1 body weight (BW)) and high (1.6 g powder kg−1 BW) doses were administered to experimental mice by intragastric gavage for 5 weeks. Changes in serum total immunoglobulin A (IgA), IgE, IgG and IgM levels, OVA-specific IgE, IgG1 and IgG2a titres and Th1/Th2 cytokine secretions using splenocytes were determined.
RESULTS: Transgenic TPY10-4 or native TCK papaya fruit supplementation did not significantly affect body, visceral organ and relative tissue weights, total IgE antibody levels, OVA-specific IgE and IgG1 antibody titres or OVA-stimulated interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-4, IL-5 and IL-10 secretions using splenocytes. However, transgenic papaya fruits markedly increased serum total IgM levels.
CONCLUSION: This study suggests that transgenic TPY10-4 papaya fruits do not increase the allergenic potential of OVA by oral administration but may have a protective immunity via increasing the serum total IgM level. Copyright © 2010 Society of Chemical Industry
Subjects
ovalbumin (OVA)-sensitised mouse model
papaya leaf distortion mosaic virus (PLDMV)
papaya ringspot virus (PRSV)
serum antibody levels
Th1/Th2 cytokines
transgenic papaya
Publisher
Wiley
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
index.html
Size
21.99 KB
Format
HTML
Checksum
(MD5):9375a21737984160c81a273959c55da8