Novel Wx alleles induced by chemical mutagens in rice, Oryza sativa L.
Journal
Plant Breeding
Journal Volume
136
Journal Issue
2
Pages
206-213
Date Issued
2017-04
Author(s)
Bing-nan Lin
Yu-chia Hsu
Su-chen Kuo
Yann-rong Lin
Yong-Pei Wu
Chie-wei Kuo
Abstract
From mutant pools of two Taiwanese elite japonica cultivars, Tainung 67 and Taikeng 8, we identified 13 mutant lines possessing opaque endosperm with relatively low amylose contents (AC) ranging from 1.5% to 7.1%. Because of different AC, paste viscosities of these 13 mutant lines differed, as revealed by palatability and physicochemical properties. The mutated gene conferring opaque endosperm was isolated from the F-2 population of one mutant line, WY1x indica cv. Taichung Sen 17', by positional cloning, revealing a G3018 -> A3018 substitution at exon 9 of Waxy leading to a non-synonymous mutation from alanine to valine. Two additional alleles were identified from the other 12 mutant lines, for which single-nucleotide substitutions G2708 -> A2708 and G3029 -> A3029 occurred in exons 8 and 9, leading to non-synonymous mutations from arginine to histidine and glutamic acid to lysine, respectively. The three novel wx alleles had different effects on grain quality, specifically on eating and cooking quality, and could be applied in rice breeding programmes to develop new low AC varieties by marker-assisted selection.
Subjects
amylose content
grain quality
opaque endosperm
physicochemical properties
waxy
Publisher
Wiley
Type
journal article