Collection, Identification, and Culturing of Phlebopus sp. from Taiwan
Other Title
臺灣脈柄牛肝菌 (Phlebopus sp.) 之採集、鑑定與培養
Journal
台灣農業研究
Journal Volume
74
Journal Issue
1
Start Page
81
End Page
85
ISSN
2790-086X
Date Issued
2025-03-07
Author(s)
Abstract
Mushrooms are rich in nutrients and popular delicacy. However, due to limitations in cultiva-tion techniques, some mushrooms that are symbiotic with plants still rely on wild harvesting. Boletes are well-known edible wild mushrooms. In 2024, samples similar to bolete mushrooms were collected in Taichung and Yunlin, Taiwan. Both fruiting bodies were medium to large in size with a dark brown pileus surface and the porous structure of hymenium. Upon vertical sectioning of the pileus, the con-text was yellow sponge-like tissue. Two isolates, designated Ph-1 and Ph-2, were isolated from these samples. To further identify the two isolates, the internal transcribed spacer (ITS) region of rDNA was amplified by polymerase chain reaction (PCR) with the primers ITS1/ITS4 and sequenced. Phy-logenetic analysis preliminarily identified both isolates as Phlebopus sp. Additionally, to investigate the optimal cultivation conditions of isolates, their colony lengths were measured on three different media including potato dextrose agar (PDA), potato rice bran (PR), and sawdust potato glucose (SPG) medium. The results showed that Ph-1 grew faster than Ph-2 on all three media, and both isolates exhibited the highest growth rate on the SPG medium. It indicated that sawdust effectively promotes the mycelial growth of Phlebopus sp. Bolete mushrooms are well-known for their unique flavor and fleshy texture. This study is the first report to identify the Phlebopus sp. isolated from Taiwan by com-bining phenotype and ITS sequence analysis. To establish commercial cultivation methods for bolete mushrooms in Taiwan, future research will focus on cultivating fruiting bodies and evaluating the fea-sibility of artificial cultivation.
菇類營養價值豐富，深受消費者喜愛。由於栽培技術之限制，部分與植物共生之菇類仍仰賴野外採集取得，牛肝菌便是其中著名可食用的野生菇類。2024 年分別於臺灣臺中市東勢區與雲林縣斗六市兩處，採集到外觀疑似牛肝菌之子實體樣本，經組織分離後暫稱為 Ph-1 與 Ph-2。兩樣本之菌傘外觀皆呈暗褐色，背面具有孔狀結構，而菌傘縱剖後，其內部構造呈淺黃色海綿狀組織。為進一步鑑定此兩菌株，本研究對其 rDNA之內轉錄區間 (internal transcribed spacer; ITS) 進行增幅與序列分析，並由親緣分析結果初步鑑定此兩菌均為脈柄牛肝菌屬 (Phlebopus sp.)。此外為開發菌株之最適培養基，以供後續人工栽培參考，利用 3 種不同之營養培養基 (potato dextrose agar (PDA)、potato rice bran (PR) 及 sawdust potato glucose (SPG)) 進行菌絲生長速度測定。結果顯示 Ph-1 之菌絲於所有培養基上生長速度皆較 Ph-2 快，且兩菌株均於 SPG 培養基上具有最佳菌絲生長速度，顯見木屑添加可有效促進脈柄牛肝菌之菌絲生長。牛肝菌以其獨特的風味與肉質口感而聞名，本篇為首次結合子實體表型與 ITS 序列分析鑑定國內脈柄牛肝菌屬之研究，未來將持續進行相關栽培試驗，同時也將評估人工栽培之可行性，以建立臺灣牛肝菌之商業化栽培方法。
Subjects
Edible bolete
Phlebopus sp.
Mycelium growth medium
可食用牛肝菌
脈柄牛肝菌屬
菌絲生長培養基
Publisher
農業部農業試驗所
Type
journal article
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