Proteomic analysis of stress-related proteins in transgenic broccoli harboring a gene for cytokinin production during postharvest senescence

Mao-Sen Liu; Hui-Chun Lia; You-Min Chang; Min-Tze Wu; Long-Fang Oliver Chen

doi:10.1016/j.plantsci.2011.06.005

Proteomic analysis of stress-related proteins in transgenic broccoli harboring a gene for cytokinin production during postharvest senescence

Journal

Plant Science

Journal Volume

181

Journal Issue

3

Pages

288-299

Date Issued

2011-09

Date

2011-09

Author(s)

Mao-Sen Liu

Hui-Chun Lia

You-Min Chang

Min-Tze Wu

Long-Fang Oliver Chen

DOI

10.1016/j.plantsci.2011.06.005

URI

https://scholars.tari.gov.tw/handle/123456789/14334

Abstract

Our previous study revealed a cytokinin-related retardation of post-harvest floret yellowing in transgenic broccoli (Brassica oleracea var. italica) that harbored the bacterial isopentenyltransferase (ipt) gene. We aimed to investigate the underlining mechanism of this delayed post-harvest senescence. We used 2D electrophoresis and liquid chromatography–electrospray ionization–mass spectrometry/mass spectrometry for a proteomics analysis of heads of ipt-transgenic and non-transgenic inbred lines of broccoli at harvest and after four days post-harvest storage. At harvest, we found an accumulation of stress-responsive proteins involved in maintenance of protein folding (putative protein disulfide isomerase, peptidyl-prolyl cis–trans isomerase and chaperonins), scavenging of reactive oxygen species (Mn superoxide dismutase), and stress protection [myrosinase-binding protein, jasmonate inducible protein, dynamin-like protein, NADH dehydrogenase (ubiquinone) Fe-S protein 1 and stress-inducible tetratricopeptide repeat-containing protein]. After four days’ post-harvest storage of non-transgenic broccoli florets, the levels of proteins involved in protein folding and carbon fixation were decreased, which indicates cellular degradation and a change in metabolism toward senescence. In addition, staining for antioxidant enzyme activity of non-transgenic plants after post-harvest storage revealed a marked decrease in activity of Fe-superoxide dismutase and ascorbate peroxidase. Thus, the accumulation of stress-responsive proteins and antioxidant enzyme activity in ipt-transgenic broccoli are most likely associated with retardation of post-harvest senescence.

Subjects

Transgenic broccoli

Isopentenyltransferase

Senescence

Stress proteins

Publisher

Elsevier Scientific Publishers Ireland Ltd.

Type

journal article

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