Identification, characterisation and detection of a new tospovirus on sweet pepper

Cheng, Y. -H.; Zheng, Y. -X.; Tai, C. -H.; Yen, J. -H.; Chen, Y. -K.; Jan, F. -J.

doi:10.1111/aab.12084

Identification, characterisation and detection of a new tospovirus on sweet pepper

Journal

Annals of Applied Biology

Journal Volume

164

Journal Issue

1

Pages

107-115

Date Issued

2014-01

Date

2014-01

Author(s)

Cheng, Y. -H.

Zheng, Y. -X.

Tai, C. -H.

Yen, J. -H.

Chen, Y. -K.

Jan, F. -J.

DOI

10.1111/aab.12084

URI

https://scholars.tari.gov.tw/handle/123456789/4007

https://onlinelibrary.wiley.com/doi/10.1111/aab.12084

Abstract

A putative virus-induced disorder showing mottling and deformation on leaves and fruits of sweet pepper was observed in Taiwan in 2009. Crude sap of symptomatic sweet pepper leaves reacted positively in enzyme-linked immunosorbent assay (ELISA) with antisera to tospoviruses in the Watermelon silver mottle virus (WSMoV) serogroup. A virus culture, TwPep3, isolated from the symptomatic sweet pepper revealed isometric virions measuring about 80-100nm in diameter via electron microscopy. Seedlings of sweet pepper inoculated with TwPep3 showed similar symptoms as those observed in the field. A 0.9-kb viral genome of isolate TwPep3 could be amplified using degenerate primers for L RNA conserved region of tospoviruses but not using those of tobamoviruses, potyviruses and Cucumber mosaic virus. The partial L RNA sequence of isolate TwPep3 (KF383955) shared 75.1% and 82.4% nucleotide identities with those of WSMoV and Tomato necrotic ringspot virus (TNRV), respectively. To determine the taxonomic relationship of isolate TwPep3, the full length of S RNA (KF383956) was sequenced. Sequence analyses showed that the nucleocapsid (N) gene of TwPep3 shared 82-83% and 87.9-89% nucleotide and amino acid sequence identities, respectively, with those of five isolates of TNRV, the most closely related tospovirus. Moreover, the NSs proteins and S RNAs of TwPep3 and TNRV shared only 78.7% and 77.6% amino acid and nucleotide sequence identities, respectively. Based on our results, isolate TwPep3 should be considered as a new tospovirus and we propose the name Pepper chlorotic spot virus (PCSV). A specific primer pair designed in the N gene was successfully used in reverse transcription-polymerase chain reaction for PCSV diagnostic work.

Subjects

Capsicum annuum

fruit deformation

mottle

sweet pepper

Tospovirus

Publisher

Wiley

Type

journal article

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