Porcine Lactoferrin Expression in Transgenic Rice and Its Effects as a Feed Additive on Early Weaned Piglets

Tzu-Tai Lee; Chi-Chung Chang; Rung-Shian Juang; Ray-Bin Chen; Hsiu-Ya Yang; Li-Wei Chu; Shih-Rong Wang; Tung-Hai Tseng; Chang-Sheng Wang; Liang-Jwu Chen; Bi Yu

doi:10.1021/jf903904s

Porcine Lactoferrin Expression in Transgenic Rice and Its Effects as a Feed Additive on Early Weaned Piglets

Journal

Journal of Agricultural and Food Chemistry

Journal Volume

58

Journal Issue

8

Pages

5166-5173

Date Issued

2010-04

Date

2010-04

Author(s)

Tzu-Tai Lee

Chi-Chung Chang

Rung-Shian Juang

Ray-Bin Chen

Hsiu-Ya Yang

Li-Wei Chu

Shih-Rong Wang

Tung-Hai Tseng

Chang-Sheng Wang

Liang-Jwu Chen

Bi Yu

DOI

10.1021/jf903904s

URI

https://scholars.tari.gov.tw/handle/123456789/1755

Abstract

The purpose of this study is to determine the growth performance and immune characteristics of early weaned piglets receiving rice bran expressing porcine lactoferrin as a feed additive. Full-length cDNA encoding porcine lactoferrin (LF) driven by a rice actin promoter was transformed into rice plants, and its integration into the rice genome was verified by Southern blot analysis. The expression of recombinant LF (rLF) in whole grains and rice bran was also confirmed, and the amount of rLF accumulated in rice bran was estimated by immunoblot assay to be approximately 0.1% of rice bran weight. An iron-binding assay showed that the rLF retained iron-binding activity and the binding capacity of 1 mg/mL rLF would be saturated by 100 μM of FeCl3. Thirty-six early weaned piglets at 21 days old were randomly selected into two groups and fed a diet containing 5% transgenic rice bran containing 50 mg/kg rLF (rLF group) and 5% rice bran (control group) to investigate the piglets’ growth performance and immune characteristics. The results showed no significant difference in growth performance between the groups during the feeding period. However, the aerobic bacteria, anaerobic bacteria, and coliform counts in the cecal contents of the rLF-fed group were significantly lower than those of the control group. Additional immune characteristics such as the IgG concentration in the rLF group was higher than the control group at the 28th day, but leukocyte counts and the peripheral lymphocyte ratio remained similar. In summary, porcine LF expressed in rice bran, a byproduct of rice, can be used as a functional additive to improve antimicrobial capabilities and IgG concentration of early weaned piglets.

Subjects

Recombinant porcine lactoferrin

transgenic rice

animal feed

growth performance

immunity characteristics

Publisher

American Chemical Society

Type

journal article

File(s)

Name

index.html

Size

21.94 KB

Format

HTML

Checksum

(MD5):133f0c898ec7b93da8b0053e2c6554ef