百合灰黴病菌的黏著現象
Other Title
Adhesive phenomena of Botrytis elliptica.
Journal
植物病理學會刊
Journal Volume
8
Journal Issue
1
Pages
15-22
Date Issued
1999-03
Author(s)
Abstract
百合灰黴病菌(Botrytis elliptica)孢子滴接在玻片上，於20 C靜置60分鐘後，約有5％孢子黏著，120分鐘達25％，160分鐘達7 5％，180分鐘以後可達80％以上。以polystyrene 和葵百合葉片( c v. Star Gazer) 作為基質時，孢子亦有相似的黏著時程。滴接後120分鐘，孢子細胞內原生質體明顯顆粒化，並開始發芽。孢子黏著在玻片表面的百分率隨著孢子發芽率的增加而增加，未發芽的孢子黏著能力較差，而孢子發芽者可緊密地黏著在物體表面上。孢子黏著作用與物體表面的疏水性無明顯關係(r=0.190. ρ＞ 0.05)。本菌孢子的發芽形式有兩種，一為短而膨大型的發芽管，一為菌絲型發芽管。基質表面疏水性與孢子產生膨大型發芽管的現象有關，表面疏水性越高，孢子產生膨大型發芽管的百分率越高。孢子發芽後，在發芽管周圍可分泌黏膜物質，以助其黏著於基質表面。孢子經過呼吸作用抑制劑疊氮化鈉、氧化丙烯或80 C熱水浴10分鐘處理後，其黏著在玻片和百合葉片上的百分率明顯下降。綜合上述試驗顯示孢子的黏著作用與孢子的代謝活性有關。
The nonspecific attachment of conidial germlings of Botrytis elliptica to various surfaces of substrata was examined. Washing experiments showed that 7% of the conidia began adhering to glass slides 60 min after dropping that was just prior to germination; after 120 min, approxminately 25% of the conidia resisted washing and remained firmly attached; not until 180 min were over 80% of conidia able to attach to the glass slide. Similar results were obtained from conidia dropped to surfaces of polystyrene and oriental lily leaf disks. The protoplasts of conidia obviously appeared granular and started germinating 120 min after inoculation at 20 C. Attachment of conidia to the substrata was increased with the increment of conidial germination. A nongerminated conidium was significantly less able than a germinated conidium to adhere to the surface of a substratum. There was no correlation (r=0.190, ρ＞0.05) between conidial adhesion and hydrophobicity of substrata. Conidia germinated with swelling and slender germ-tubes were observed. The hydrophobic substrata induced production of a swelling in germ-tubes from conidia. Light microscopy and SEM observations showed that an ensheathing material secreted from conidia and germ tubes appeared around the germlings of B. elliptica. Attachment of conidia was prevented by metabolic inhibitors such as sodium azide, propylene oxide and 80℃-hot water. The results suggested that metabolic activity of the pathogen was required for conidial adhesion.
Subjects
百合灰黴病菌
黏著作用
菌絲鞘
代謝活性
疏水性
Botrytis elliptica
adhesion
hyphal sheath
metabolic activity
hydrophobicity
Publisher
中華民國植物病理學會
Type
journal article