香水百合"Casa Blanca"莖頂培養之大量繁殖
Other Title
Mass Propagation of Oriental Lily “Casa Blanca” by Shoot Tip Culture
Journal
中華農業研究
Journal Volume
41
Journal Issue
2
Pages
169-177
Date Issued
1992-06-05
Date
1992-06-05
Author(s)
紀海珊
葉常青
H.S. Gi
C.C. Yeh
Abstract
香水百合之鱗莖,剝去其外圍之鱗片,取約300~400μm含1對葉原體之莖頂,培養於含有0.2ppm NAA AA、4ppm BA、3%蔗糖及0.8%洋菜之Murashige and Skoog (1962)(MS)基本鹽類培養基中,經2個月的培養,可形成約1.5cm大小之原球體,此原球體培養於含有0.5ppm NAA、4ppm BA、3%蔗糖及0.8%洋菜之MS基本鹽類培養基中可行原球體之增殖;而培養於含有0.2ppm 2,4-D、0.5ppm BA、3%蔗糖及0.8%洋菜之MS基本鹽類培養基中則可誘導植株形成。Mass propagation of Oriental lily by shoot tip culture was developed for the purpose of accelerating the propagation rate and avoiding the transmission of virus. Bulb scales of ”Casa Blanca” containing 1 pair of leaf primordia, measuring about 300-400 im in size, were excised from the bulb and cultrued. The composition of the medium was MS salts supplemented with 0.2 ppm NAA, 4 ppm BA, 3% sucrose and 0.8% phytagar. Protocorms about 1.5 cm in diameter could be produced after 2 months in culture.
Subjects
香水百合
大量繁殖
CaSa blanca
Mass propagation
Publisher
農業試驗所
Type
journal article
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