秋水仙素處理對藍紫色蝴蝶蘭Phalaenopsis pulcherrima fma. coerulea 原球體多倍體誘導之影響
Other Title
Effects of Colchicine Treatments on Polyploidy Induction Using Protocorms of Phalaenopsis pulcherrima fma. coerulea
Resource
台灣農業研究 67(3): 247-257
Journal of Taiwan Agricultural Research 67(3): 247-257
Journal
台灣農業研究
Journal Volume
67
Journal Issue
3
Pages
247-257
Date Issued
2018-09-02
Date
2018-09-02
Author(s)
曹進義
陳威臣
吳容儀
吳姿穎
夏奇鈮
Chin-Yi Tsao
Uei-Chern Chen
Rung-Yi Wu
Tzu-Ying Wu
Chi-Ni Hsia
Abstract
本研究以藍紫色原生種蝴蝶蘭Phalaenopsis pulcherrima fma. coerulea無菌播種發芽後約6 wk齡之原球體(protocorm)作為培植體,探討秋水仙素(colchicine)濃度及處理時間對多倍體誘導之影響,並以流式細胞儀與根尖染色檢測苗株之多倍性。
Effects of colchicine concentration and exposure time on polyploidy induction, using 6-wk-old protocorms of Phalaenopsis pulcherrima fma. coerulea, were conducted in this study. Ploidy levels of seedlings derived from colchicine treatments were analyzed by flow cytometry and chromosome counting of root tips.
Subjects
蝴蝶蘭
原球體
秋水仙素
倍體數
流式細胞儀
Phalaenopsis
Protocorm
Colchicine
Polyploidy
Flow cytometry
Publisher
農業試驗所
Type
journal article
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