彩虹鳥蕉組織培養之研究 I. 培植體離體培養污染細菌之檢定及防治
Other Title
Studies on Tissue Culture of Heliconia psittacorum ‘Rhizomatosa’ I. Identification and Control of Bacterial Contamination of Explants Cultured In Vitro
Journal
中華農業研究
Journal Volume
47
Journal Issue
4
Pages
346-363
Date Issued
1998-12-03
Date
1998-12-03
Author(s)
Abstract
本試驗以彩虹烏蕉Heliconia psttacorum Rhizomatosa'為材料，進行植株各部位培植體之離體培養，嘗試篩檢污染菌類，並尋求降低高污染率的方怯。結果顯示，離體培養21天後各部位培植體皆有高污染率，其中以根莖新芽污染率100豔最高，葉片上半部(57.7%)較低，葉片下半部(83.3%)、葉柄(80%)、子房(90%)和花梗(96.7%)之污染率也都在八成以上；除子房外，其它培植體受真菌類污染比例偏低，污染源以細菌性為主，且全部為革蘭氏陰性。
This study were to identify and control the contaminants in tissue culture of Heliconia psittacorum 'Rhizomatosa'. Explants from different parts of H. psittacorum 'Rhizomatosa' were tested for the percentage of contamination. The lowest contamination rate was obtained from leaf tip explants (57.7%) after 21 days of culture, and 80%, 83.3%, 90%, 96.7%, 100% contamination for petiole, leaf base, ovary, peduncle and terminal bud, respectively. Six species of bacteria were isolated and characterized from explants of H. psittacorum 'Rhizomatosa'.
Subjects
彩虹鳥蕉
離體培養
培植體
細菌檢定
Heliconia psittacorum
In vitro
Explant
Bacterial identification
Publisher
農業試驗所
Type
journal article
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