花椰菜雙單倍體誘變平台建立
Other Title
Establishment of a Doubled Haploid Mutagenesis Platform in Cauliflower
Journal
台灣農業研究
Journal Volume
70
Journal Issue
3
Pages
182-195
Date Issued
2021-09-03
Author(s)
夏奇鈮
陳威臣
許秀惠
曹進義
Chi-Ni Hsia
Uei-Chern Chen
Shiow-Huey Hseu
Chin-Yi Tsao
Abstract
本研究採用雙單倍體 (doubled haploid; DH) 作為小孢子供體，於小孢子培養過程中施以誘變處理，建立花椰菜雙單倍體誘變平台。誘變處理包括以紫外光 (ultraviolet; UV) 處理4 個不同結球天數花椰菜DH 品系之小孢子，DH 品系源自「慶農S-65」小孢子培養；以及利用ethyl methanesulphonate (EMS) 處理上述UV 試驗所得之小孢子胚，並將上述兩誘變處理所得DH 植株種植於田間，觀察誘變株發育階段的變化。
An integrated haploid mutagenesis platform through doubled haploid (DH) donor plants providing microspores in cauliflowers was established in this study. Ultraviolet (UV) irradiation was applied to microspores of four DH lines derived from ‘Chinglong S-65’ and each line with various heading mature days. Embryos derived from UV treated microspores of DH32 as stated above were treated with ethyl methanesulphonate (EMS) subsequently for double mutagenesis. EMS was also applied on flower buds of ‘Chinglong S-65’ before microspore culturing for mutagenesis.
Subjects
花椰菜
小孢子培養
雙單倍體
單倍體誘變
Brassica oleracea var. botrytis L.
Microspore culture
Doubled haploid
Haploid mutagenesis.
Publisher
農業試驗所
Type
journal article
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