Compositional and Proteomic Analyses of Genetically Modified Broccoli (Brassica oleracea var. italica) Harboring an Agrobacterial Gene

Mao-Sen Liu; Miau-Hwa Ko; Hui-Chun Li; Shwu-Jene Tsai; Ying-Mi Lai; You-Ming Chang; Min-Tze Wu; Long-Fang O. Chen

doi:10.3390/ijms150915188

Compositional and Proteomic Analyses of Genetically Modified Broccoli (Brassica oleracea var. italica) Harboring an Agrobacterial Gene

Journal

International Journal of Molecular Sciences

Journal Volume

15

Journal Issue

9

Pages

15188-15209

Date Issued

2014-09

Date

2014-09

Author(s)

Mao-Sen Liu

Miau-Hwa Ko

Hui-Chun Li

Shwu-Jene Tsai

Ying-Mi Lai

You-Ming Chang

Min-Tze Wu

Long-Fang O. Chen

DOI

10.3390/ijms150915188

URI

https://scholars.tari.gov.tw/handle/123456789/6689

https://www.mdpi.com/1422-0067/20/19/4879

Abstract

Previously, we showed improved shelf life for agrobacterial isopentenyltransferase (ipt) transgenic broccoli (Brassica oleracea var. italica), with yield comparable to commercial varieties, because of the protection mechanism offered by molecular chaperones and stress-related proteins. Here, we used proximate analysis to examine macronutrients, chemical and mineral constituents as well as anti-nutrient and protein changes of ipt-transgenic broccoli and corresponding controls. We also preliminarily assessed safety in mice. Most aspects were comparable between ipt-transgenic broccoli and controls, except for a significant increase in carbohydrate level and a decrease in magnesium content in ipt-transgenic lines 101, 102 and 103, as compared with non-transgenic controls. In addition, the anti-nutrient glucosinolate content was increased and crude fat content decreased in inbred control 104 and transgenic lines as compared with the parental control, "Green King". Gel-based proteomics detected more than 50 protein spots specifically found in ipt-transgenic broccoli at harvest and after cooking; one-third of these proteins showed homology to potential allergens that also play an important role in plant defense against stresses and senescence. Mice fed levels of ipt-transgenic broccoli mimicking the 120 g/day of broccoli eaten by a 60-kg human adult showed normal growth and immune function. In conclusion, the compositional and proteomic changes attributed to the transgenic ipt gene did not affect the growth and immune response of mice under the feeding regimes examined.

Subjects

allergen

Brassica oleracea var. italica

broccoli

proteomics

glucosinolate

isopentenyltransferase (ipt)

Publisher

MDPI

Type

journal article

File(s)

Name

index.html

Size

21.92 KB

Format

HTML

Checksum

(MD5):d6c451fa9051e8e53d25fba5985507b6