作物螺旋線蟲(spiral nematode)病害之診斷鑑定

Other Title
The diagnosis and identification of the spiral nematode associated with crop disease
Journal
植物重要防檢疫疫病害診斷鑑定技術研習會專刊(六)
Pages
9-24
ISBN
978-986-01-0647-3
Date Issued
2007-09
Date
2007-09
Author(s)
陳殿義
Chen, D. Y.
URI
Abstract
植物病原線蟲中所泛稱的螺旋線蟲（spiral nematode）包括Helicotylenchus Steiner, 1945、 Scutellonema Andrassy, 1958及Rotylenchus Filipjev, 1936等三個屬之線蟲，其中最重要的檢疫線蟲種類為H. multicinctus (Cobb, 1893) Golden, 1956和S. bradys (Steiner and LeHew, 1933) Andrássy, 1958，其分別會造成香蕉和山藥作物的嚴重病害。截至目前，在台灣和金門地區，從13類作物的根圈土壤中，以改良式柏門氏漏斗分離法，共計分離到28群螺旋線蟲，經以光學和SEM的形態鑑定及輔以rDNA片段鹼基序列相似度的分析，其中9群被鑑定為Helicotylenchus dihystera (Cobb, 1893) Sher, 1961、6群為H. crenacauda Sher, 1966、4群為Rotylenchus brevicaudatus Colbran, 1962、6群為Scutellonema brachyurum (Steiner, 1938) Andrássy, 1958、3群為S. truncatum Sher, 1963。上述5種螺旋線蟲，其中 R. brevicaudatus至目前僅在金門地區發現，而S. brachyurum和S. truncatum為台灣地區的新記錄種。至於rDNA片段的序列大小，前述5種螺旋線蟲依序分別為1317 bp、1316 bp、1222 bp、1343 bp及1457 bp，並為首次在GenBank序列資料庫中登錄之。經由較多供試群的形態測量值和rDNA片段序列相同度的綜合比對分析，對台灣地區此5種螺旋線蟲的形態變異範圍已有了更確切的認知。今後開發正確、快速及簡便之線蟲種類分生鑑定技術，將能更落實植物線蟲防檢疫工作的推動 The common name, spiral name, includes three genus plant-parasitic nematodes, Helicotylenchus (Steiner, 1945), Scutellonema (Andrássy, 1958) and Rotylenchus (Filipjev, 1936). Two of important quarantine nematodes are H. multicinctus (Cobb, 1893) Golden, 1956 and S. bradys (Steiner and LeHew, 1933) Andrássy, 1958. They can cause serious disease of banana and yam respectively. 28 groups of spiral nematode have isolated form 13 crops soil in Taiwan and Kinmen area by modified Baermann tray method. Morphological identification of optical microscope and SEM observation and analysis of rDNA fragment have been used. The result show that 9 groups are belonged to H. dihystera (Cobb, 1893) Sher, 1961, 6 groups are H. crenacauda Sher, 1966, 4 groups are R. brevicaudatus Colbran, 1962, 6 groups are S. brachyurum (Steiner, 1938) Andrássy, 1958, 3 groups are S. truncatum Sher, 1963. R. brevicaudatus is only appeared in Kinmen region and S. brachyurum and S. truncatum are new record in Taiwan. The representative sequences of rDNA fragment for these five species had the size of 1317 bp, 1316 bp, 1222 bp, 1343 bp, and 1457 bp respectively, and which were the first submitted to the GenBank.
Subjects
螺旋線蟲
鑑定
診斷
Helicotylenchus dihystera
Helicotylenchus crenacauda
Scutellonema brachyurum
Scutellonema truncatum
Rotylenchus brevicaudatus
核糖體DNA
spiral nematode
identification
diagnosis
Helicotylenchus dihystera
Helicotylenchus crenacauda
Rotylenchus brevicaudatus
Scutellonema brachyurum
Scutellonema truncatum
ribosomal DNA
Publisher
行政院農委會動植物防疫檢疫局、國立中興大學植物病理系
Type
book chapter
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