超高壓液相層析儀快速分析台灣蔬菜葉黃素之新方法
Other Title
An Improved Ultra Performance Liquid Chromatography Method for Analyzing Lutein Content of Vegetables in Taiwan
Journal
台灣農業研究
Journal Volume
63
Journal Issue
2
Pages
159-166
Date Issued
2014-06-07
Date
2014-06-07
Author(s)
Abstract
葉黃素可延緩眼睛老化及黃斑部病變，但人體無法自行合成，需由飲食中補充。為建立快速分析葉黃素之方法與台灣常見市售蔬菜葉黃素含量之資料庫，本研究利用超高壓液相層析儀，使用管柱ethylene bridged hybrid（BEH）C18，以氰甲烷和甲醇（70:30）之混合液為移動相，等位沖提流洗下，可在5 min之內定量標的物葉黃素。
Lutein can mitigate eyes' aging and macular degeneration, but cannot be synthesized in the human body. It must be supplemented from diet. An efficient method for separation and quantification of lutein was developed to establish the database of lutein contents of various commercial vegetables in Taiwan. By ultra performance liquid chromatography, lutein can be analyzed within 5 min using ethylene bridged hybrid (BEH) C18 as a column and an isocratic elution solution of acetonitrile/ methanol (70:30, v/v) at a flow rate 0.25 mL min^(-1).
Subjects
葉黃素
蔬菜
超高壓液相層析儀
Lutein
Vegetables
Ultra performance liquid chromatography
Publisher
農業試驗所
Type
journal article
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