芥菜抗蕪菁嵌紋病毒與同功異構酶伴連之研究

Other Title
Associated Analysis of Isozymes and Turnip Mosaic Virus (TuMV) Resistance in Mustard (Brassica Juncea (L.) Czerniak.)
Journal
中國園藝
Journal Volume
38
Journal Issue
3
Pages
149-160
Date Issued
1992-09
Date
1992
Author(s)
張有明
許圳塗
蕭吉雄
林正忠
Y.M. Chang
C.T. Shii
C.H. Hsiao
C.C. Lin
DOI
URI
Abstract
以抗蕪菁嵌紋病毒(TuMV)的苗栗芥菜（R型）和感病的新竹、竹北芥菜（S型）進行七種酵素比較分析；三品種間GOT酵素個體間條帶型均一致沒有差異，基酶圖呈單態型。SkDH和PRX在抗病品系(R)與感病品系(S)有一處不一樣的條帶型，而ACP-α-5群有二處相異的條帶型。後裔檢定以抗病品系（苗栗）感病品系（竹北）的雜交F1及F2代族群，分析ACP，PRX和SkDH之變異性，在雜交F1代各個體中三種酵素表現型（F1型）皆很一致，具表現兩親本型的共顯性現象。F2子代在差異區ACP-α-5F、ACP-α-5S、SkDH-1及PRX-1的分離顯示這些異型酶爲單元體，由後裔分析，知四個相異區爲單一因子遺傳；其基因座分別命名爲Acp-α-5F、Acp-α-5S、Skdh-1和Prx-1,可提供作爲遺傳指標。兩參試品系經接種調查F1、F2代之發病情形，並分析病徵與四個酵素基因表現型之關係；F1代大都均呈現抗、耐病性，顯示抗病性有顯性因子參予；F2分離族群病徵分四群：無病徵（抗病）、脈透明化（耐病）、嵌紋病（感病）和嚴重嵌紋狀（嚴重感病），並分析ACP-α，PRX和SkDH的表現型顯示ACP-α-F，ACP-α-S，SsDH-1和抗病性無相關性，而PRX-1之抗病型（R型），出現40%的抗病株和55%的耐病株，初步顯示，PRX-1和抗病性有關。Prx-1和抗病基因有伴連作用。 The inheritance of ACP-α, GOT, PRX and SkDH isozymes between ”Chu-Pei”, ”Shin-Chu” (susceptible of TuMV) and ”Miau-Li” (resistant to TuMV) mustard were analyzed through progeny testing. Monomorphism of GOT phenotype in three mustards were detected. Since ”Chu-Pei” and ”Shin-Chu” have same band pattern of four isozymes, the progeny test only involved two varieties (Miau-Li and Chu-Pei). The F1 from of ACP-α, PRX and SkDH were characterized by the codominance expression of parental types. Two parental types were recovered in F2 population. The segregation of phenotypes fit a 3:1 ration, demonstrating a monofactor inheritance. It is suggested that null allele of PRX-1 was existing in ”Miau-Li” (R type), and that of ACP-α-5S and SkDH-1 in ”Chu-pei” (S type) mustard. Three isozymes are tentatively assigned to be controlled by Acp-α-5F, Acp-α-5S, Prx-1 and Skdh-1 loci respectively. The result to TuMV was evaluated in ”Miau-Li”, ”Chu-Pei” and their progenies by inoculated test and it is relationship with 4 loci were also analysed. Resistance seem to be specified by dominant genes through F1 generation expression. In F2's judged were by divided into 4 groups including symptomless (resistant), vein clearing (tolerant), mosaic (susceptible) and severe mosaic (serious susceptible). Analysis result from phenotypes of 4 isozymes loci indicated the relationship of resistance (or tolerance) with PRX-1 only. More than 95% of PRX-1 ”R type” was detected in F2 population. It is postulated that Prx-1 loci is association TuMV resistant gene, can be used as genetic marker for TuMV reistance.
Subjects
芥菜
蕪菁嵌紋病毒
同功異構酶
伴連
mustard
turnip mosaic virus TuMV
isozyme
associate
Publisher
臺灣園藝學會
Type
journal article
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