Somatic embryo formation and germination from immature embryo-derived suspension-cultured cells of Angelica sinensis (Oliv.) Diels

H. S. Tsay; H. L. Huang; H. S. Tsay;H. L. Huang

doi:10.1007/s002990050463

Somatic embryo formation and germination from immature embryo-derived suspension-cultured cells of Angelica sinensis (Oliv.) Diels

Journal

Plant Cell Reports

Journal Volume

17

Journal Issue

9

Pages

670-674

Date Issued

1998-06

Date

1998-06

Author(s)

H. S. Tsay

H. L. Huang

DOI

10.1007/s002990050463

URI

https://scholars.tari.gov.tw/handle/123456789/1772

Abstract

Embryogenic callus was induced from immature embryos of Angelica sinensis cultured on Murashige and Skoog (MS) basal medium. Embryogenic callus growth was more rapid on MS basal medium than on B5 or White medium. Embryogenic callus was used to establish a suspension culture and somatic embryos and germinating embryos developed during the culture. A shaking speed of 80 rpm was found to be optimal for establishing suspension cultures, while. 100 rpm produced more somatic embryos and germinating embryos with an initiation cell density of 0.2 ml packed cell volume/25 ml medium. Adding 0.3% agar to the liquid medium also stimulated the formation of somatic and germinating embryos. While no plant growth regulators were needed for culture initiation and plant regeneration, the addition of 0.5-1 mg/l 2,4-dichlorophenoxyacetic acid was needed to maintain the embryogenic suspension culture by preventing embryo germination. Forty percent of the germinating embryos survived after culturing on filter paper moistened with liquid half-strength MS medium containing 3% sucrose. The plants were successfully transferred into soil.

Subjects

Angelica sinensis

immature embryo culture

somatic embryo

suspension cell culture

Publisher

Springer International

Type

journal article

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