分子生物技術在藥用植物之應用研究
Other Title
Applications of Biotechniques to the Medicinal Plants
Start Page
73
End Page
86
ISBN
957-02-4205-1
Date Issued
1999-03
Date
1999-03
Author(s)
鄭可大
劉新裕
蔡新聲
Kur-Ta Cheng
Sin-Yie Liu
Hsin-Sheng Tsay
Abstract
藥用植物資源的開發與利用，為我國加入GATT後，發展精緻農業重要的一環。其種源庫的建立，目前已初具成果。惟收集品種繁多，為了有效釐清藥用植物的基原，分子生物技術實為一準確的方法。本研究利用快速且簡便的逢機擴增多型性去氧核醣核酸（RAPD）指紋分析技術，針對金線蓮、山藥及柴胡，篩選各品種（系）的特異性遺傳標誌，定其親源關係，以作為今後育種的參考。同時，以差異性表現等生物技術，尋找影響活性成份生合成的基因，作為轉殖的研究，現在正積極著手中。除此之外，中藥材的DNA鑑定，亦以RAPD及PCR-SSCP技術，進行黃連、黃耆、冬蟲夏草、當歸、枸杞及柴胡的分析。
The development and utilization of medicinal plants as resources is one of important strategies in Taiwan after the admission of the General Agreement on Traiffs and Trade (GATT). The germplasmic bank of medicinal plants has been well established during the past two years. Molecular biological techniques are reliable approaches to clarify the taxonomic levels of the medicinal plants. In our studies, a rapid and sensitive tool of random amplified polymorphic DNA (RAPD) technique was employed to construct the phylogenic relationships among the collected plant species and to screen species-specific (or line-specific) markers of some Anoectochilus species, Dioscorea species, or Bupleurum species. In addition, for transgenic purpose, differential display technique was also adapted to obtain the gene which is involved in the biosynthesis pathway of the active compound. On the other hand, the authentication of dried Chinese herbal medicines, namely, Rhizoma Coptidis, Radix Astragali, Cordyceps, Radix Angelica Sinensis, Fructus Lycii, and Radix Bupleuri, were performed by using the RAPD and the PCR (polymerase chain reaction) - SSCP (single strand conformation polymorphism) techniques.
Event(s)
1999藥用植物之開發與利用研討會
Proceedings of the Symposium on Development and Utilization of Medicinal Plants 1999
Publisher
農業試驗所
Type
book part
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