De Novo Regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and Quantitative Analysis of Harpagoside, an Iridoid Glucoside, Formed in Aerial and Underground Parts of In Vitro Propagated and Wild Plants by HPLC

Journal
Biological & Pharmaceutical Bulletin
Journal Volume
24
Journal Issue
11
Pages
1311-1315
Date Issued
2001-11
Date
2001-11
Author(s)
Abhay P. Sagare
Chao-Lin Kuo
Fu-Shin Chueh
Hsin-Sheng Tsay
DOI
URI
Abstract
A protocol for de novo regeneration and rapid micropropagation of Scrophularia yoshimurae (Scrophulariaceae) has been developed. Multiple shoot development was achieved by culturing the shoot-tip, leaf-base, stem-node and stem-internode explants on Murashige and Skoog (MS) medium supplemented with 4.44 μM N6-benzyladenine (BA) and 1.07 μM α-naphthaleneacetic acid (NAA). Stem-node and shoot-tip explants showed the highest response (100%) followed by stem-internode (74.4%) and leaf-base (7.7%) explants. The shoots were multiplied by subculturing on the same medium used for shoot induction. Shoots were rooted on growth regulator-free MS basal medium and the plantlets were transplanted to soil and acclimatized in the growth chamber. The content of harpagoside, a quantitatively predominant iridoid glycoside, in different plant material was determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of harpagoside in the aerial and underground parts of S. yoshimurae was significantly higher than the marketed crude drug (underground parts of Scrophularia ningpoensis).
Subjects
germplasm conservation
harpagoside
Scrophularia yoshimurae
Scrophularia ningpoensis
Scrophulariaceae
micropropagation
Publisher
Pharmaceutical Society of Japan
Type
journal article
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