提高茄子種原發芽率初探
Other Title
Study on the Improvement of Seed Germination of Eggplant Germplasm
Journal
台灣農業研究
Journal Volume
74
Journal Issue
4
Start Page
515
End Page
524
ISSN
2790-086X
Date Issued
2025-12-09
Author(s)
Abstract
茄子 (Solanum melongena L.) 為重要蔬菜亦為國家作物種原庫選定之核心種原，本研究以種原庫發芽率 (1%) 低下之茄子種原進行紙上法播種，比較浸種時間 (8、16 及 24 h)、溫湯處理 (55℃，15 min) 及培養溫度 (27℃恆溫或 30℃，16 h/20℃，8 h 變溫 ) 對發芽之影響，結果顯示 16 h 浸種可加速發芽，配合變溫培養在播種 2 wk 後之發芽率最高為 4.0%。將上述播種 2 wk 後未發芽之種子移至含有 0–200 mg L-1 GA3 之 1/2 倍濃度MS 培養基 (Murashige and Skoog medium) 中，並維持其原培養溫度條件，結果顯示在未添加 GA3 之 1/2 MS培養基中以恆溫培養 3 wk 後發芽率達 12.5%，而在添加 100 mg L-1 GA3 培養基配合變溫培養處理中發芽率可達 25.0%。接續測試 0、1/2、1 倍濃度 MS 基礎鹽類作為培養液與添加 0、50 mg L-1 GA3 之組合處理對種子發芽的影響，結果顯示 MS 與 GA3 兩處理對發芽 (定義為胚根突出 2 mm) 的影響並不顯著，但 1/2 MS 或 50 mg L-1 GA3 單獨處理皆有助發芽種子上胚軸芽體的生長，對後續小苗生長發育具有提升效果。
Eggplant (Solanum melongena L.) is an important vegetable and a core collection of the Na-tional Plant Genetic Resources Center in Taiwan. Seeds of eggplant with a low germination rate (1%) in the seed bank stock were used in this study. Effects of immersion duration (8, 16, and 24 h), hot water treatment (55℃ for 15 min), and culture temperature (27℃ for 24 h, and 30℃ for 16 h/20℃ for 8 h) on seed germination were investigated utilizing the paper germination method. Results showed that among the three factors, only immersion duration significantly increased the seed germination 2 wk after sowing, and the highest germination rate of 4.0% was obtained with immersion for 16 h with variable temperature culture (30℃/20℃, 16 h/8 h). The above-mentioned ungerminated seeds 2 wk after sowing were transplanted into a 1/2 MS medium (Murashige and Skoog medium) supplemented with 0–200 mg L-1 GA3 and consistently cultured under the same temperature conditions, which were either constant 27℃ or variable temperature conditions. The results showed that 1/2 MS culture medium without GA3 had a 12.5% germination rate after 3 wk of culture. Furthermore, an increased germination rate of 25.0% was achieved by combining 1/2 MS culture medium with 100 mg L-1 GA3 and cultured in variable temperature conditions. Treatments in combination with MS basal salt concentration (0, 1/2, and 1 strength) and GA3 concentration (0 and 50 mg L-1) in the sowing culture solution were tested successively. Although the results showed that the two factors had no significant effect on seed germination (based on radicle protrusion 2 mm), enhanced shoot germination rates were observed on the treatments with either 1/2 MS or 50 mg L-1 GA3 alone.
Subjects
茄子
種子
發芽
激勃素
MS 培養基
Solanum melongena L.
Seed
Germination
Gibberellic acid
MS medium
Publisher
農業部農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
74-4-9.pdf
Size
1.8 MB
Format
Adobe PDF
Checksum
(MD5):2367e0b196a038302f5c8d825b96b5e1