Evaluation of the Use of One-Step Quantitative RT-PCR for Detection of Potato Virus X
Other Title
使用單步驟定量RT-PCR法於馬鈴薯X病毒檢測之評估
Journal
台灣農業研究
Journal Volume
74
Journal Issue
2
Start Page
177
End Page
187
ISSN
2790-086X
Date Issued
2025-06-07
Author(s)
農業部農業試驗所
劉雅婷
農業部農業試驗所
農業部農業試驗所
農業部農業試驗所
鳳山熱帶園藝試驗分所
Abstract
This study developed a real-time reverse transcription-polymerase chain reaction (RT-PCR) de-tection method for potato virus X (PVX) by designing a specific primer pair based on the coat protein (CP) gene. The assay demonstrated a sensitivity approximately 10–100 times higher than convention-al RT-PCR for PVX detection. Furthermore, it exhibited high specificity, accurately detecting PVX without cross-reacting with other solanaceous viruses or uninfected healthy plants. The reliability of this method was further confirmed through field testing on PVX-infected potato samples. Due to its high sensitivity and specificity, the real-time RT-PCR assay developed in this study has significant po-tential for the early detection of PVX infections in potatoes and tomatoes.
本研究依據馬鈴薯 X 病毒 (potato virus X; PVX) 之鞘蛋白 (coat protein) 基因設計專一性引子對，利用即時反轉錄聚合酶連鎖反應 (real-time reverse transcription-polymerase chain reaction; real-time RT-PCR) 與 TaqMan螢光探針，建立馬鈴薯 X 病毒的檢測技術。本研究所開發之 real-time RT-PCR 法的靈敏度，在檢測 PVX 上相較於 RT-PCR 法，提高約 10–100 倍，此外，此檢測具有高度特異性，可成功檢測 PVX，且不會與其他茄科病毒或未感染的健康植物發生交叉反應。透過檢測田間感染 PVX 的馬鈴薯，進一步確認此檢測方法的穩定性。有鑑於 real-time RT-PCR 的高特異性和敏感性，本研究所開發的 real-time RT-PCR 檢測在評估馬鈴薯或番茄受 PVX 感染的早期檢測具有高度應用效益。
Subjects
Potato virus X
Real-time RT-PCR
Detection
馬鈴薯X病毒
即時定量 RT-PCR
偵測
Publisher
農業部農業試驗所
Type
journal article
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