水稻白葉枯病菌病原型之研究
Other Title
Pathotypes of Xanthomonas campestris pv. oryzae in Taiwan
Journal
中華農業研究
Journal Volume
31
Journal Issue
4
Pages
321-333
Date Issued
1982-12-08
Date
1982-12-08
Author(s)
廖英明
簡錦忠
Y.M. Liao
C.C. Chien
Abstract
由本省北、中、南部分離得之72 個水稻白葉枯病菌菌株，利用五種日本判別品種（Kinmaze , Kogyoku , Chugoku 45 , Rantai-Emas 及Java 14 ）的抗或感性，可分為四種病原型；病原型I ，包括16 個菌株，僅Chugoku45 呈抵抗性；病原型II，包括34 個菌株，五個水稻品種之反應均為感染性；病原型III，包括13 個菌株，僅Kogyoku 為抵抗性；病原型IV，包括9 個菌株，僅Java 14 為抵抗性。若由五種菲律賓判別品種（IR 8 , IR 20 , IR 1545-339 , DV 85 , Cas 209 ）亦可將72 個菌株區分為四種病原型；病原型I ，包括18 個菌株，IR20 及IR 1545-339 二品種之反應為抵抗性；病原型II，包括8 個菌株，僅IR 1545-339 為抵抗性；病原型III，包括42 個菌株，五個水稻品種反應均為感染性；病原型IV，包括4 個菌株，僅DV 85 為抵抗性，而以十種判別品種綜合區分時，則可將72 個菌株分為13 種病原型。72 個菌株的20 種生理生化特性試驗發現各菌株間並沒有明顯的生化型存在，但在過氧化氫酶與精氨酸雙水解酶之產生，及鹼性甲基紅與Voges-proskauer 測定，稍有不同。比較剪葉接種與針刺接種法時，發現針刺接種法較能產生典型的病徵，而剪葉接種法則適用於田間大量接種，二者在區分病原型之結果上並無差異。
In order to verify the existance of pathogenic type of Xanthomonas campestris pv. oryzae in Taiwan, 72 isolates were collected from southern, central and northern Taiwan in 1981 and tested on Japanese set and Philippine set of rice differential cultivars. Four pathotypes were identified from the Japanese set of five differentials; Pathotype I, including 16 isolates, only Chugoku 45 was resistant; Pathotype II, 34 isolates, infects all 5 Japan differentials; Pathotype III, only Kogyoku was resistant; Pathotype IV, 9 isolates, only Java 14 was resistant. According to the Philippine set of differentials, 18 isolates were classified as pathotype I, IR 20 and IR1545-339 were resistant, 8 isolates as pathotype II only IR 1545-339 was resistant, 42 isolates as pathotype III which infects all 5 Philippine differentials; 4 isolates as pathotype IV only DV 85 was resistant. Thirteen pathtypes were identified from the 10 Japanese plus philippine set of differentials,
The results of 20 physiological and biochemical characteristics of the 72 isolates of X. campestris pv. oryzae revealed that no district biochemical groups were observed. All isolates used in this study had the same reaction in 16 tests, but different in the other 4 tests. The prodution of catalase and Arginine dihydrolase, Methylene red and Voges-pro skauer test were observed. No. relationship was recorded between physiological and/or biochemical characteristics and pathogenicity. In the comparison of inoculation method, Pin-prick is more suitable for producing typical symptoms in pathogenicity detection but leaf clipping is convenient for mass inoculation in the field. However, these two inoculation methods did not affect the classification of pathotypes.
Publisher
農業試驗所
Type
journal article
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