Cordyceps militaris Reduces Oxidative Stress and Regulates Immune T Cells to Inhibit Metastatic Melanoma Invasion

Yuan-Hong Lan; Yun-Sheng Lu; Ju-Yu Wu; Hsu-Tung Lee; Penjit Srinophakun; Gizem Naz Canko; Chien-Chih Chiu; Hui-Min David Wang

doi:10.3390/antiox11081502

Cordyceps militaris Reduces Oxidative Stress and Regulates Immune T Cells to Inhibit Metastatic Melanoma Invasion

Journal

Antioxidants

Journal Volume

11

Journal Issue

8

Pages

1502

Date Issued

2022-08

Author(s)

Yuan-Hong Lan

Yun-Sheng Lu

Ju-Yu Wu

Hsu-Tung Lee

Penjit Srinophakun

Gizem Naz Canko

Chien-Chih Chiu

Hui-Min David Wang

DOI

10.3390/antiox11081502

URI

https://www.mdpi.com/2076-3921/11/8/1502

https://scholars.tari.gov.tw/handle/123456789/17771

Abstract

In this study, the water extract of Cordyceps militaris (Linn.) Link (CM) was used as a functional material to investigate the inhibitory mechanisms on B16F10 and lung metastatic melanoma (LMM) cells. Reducing power, chelating ability, and 2,2-diphenyl-2-picrylhydrazyl (DPPH) assays were applied for antioxidative capacities, and we obtained positive results from the proper concentrations of CM. To examine the ability of CM in melanoma proliferation inhibition and to substantiate the previous outcomes, three cellular experiments were performed via (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT, a tetrazole) assay, cell migration, and invasion evaluation. The addition of CM to the incubation medium increased the number of CD8+ T cells significantly, which improved the immunogenicity. This study showed that CM exhibits various biological capabilities, including antioxidation, anti-tumor, tumor invasion suppression, and T cytotoxic cell activity promotion.

Subjects

Cordyceps militaris

lung metastatic melanoma

tumor microenvironment

T cell

Publisher

MDPI

Type

journal article