白菜細菌性軟腐病生物防治潛力菌株的篩選與評估

由Pectobacterium carotovorum subsp. carotovorum (Pcc) 引起之細菌性軟腐病為白菜常見病害之一，本研究目的為篩選並評估具防治本病害潛力的微生物。利用離葉接種法評估6株拮抗能力較佳之Bacillus spp. 菌株AC35、BAC15、NA0301、HTT-11、HTT-61及HTT-62對軟腐病之防治效果，結果顯示不管使用針刺接種法或是噴霧接種法，僅接種軟腐病菌Pcc10602之處理組於接種後第5天罹病度達100%，而供試菌株BAC15、HTT-61及HTT-62對軟腐病具有防治效果，其罹病度分別為52.4%、57.1%及57.1%。溫室試驗時，利用棉花包覆法與噴霧接種法於白菜上處理Bacillus amyloliquefaciens菌株細胞懸浮液 [濃度108 colony-forming unit (CFU) mL-1]，1 d 後再接種Pcc10602 (濃度105 CFU mL-1)，結果顯示對照組用兩種不同方式接種後12 d的罹病度分別為97.9% 與74.5%；而HTT-62菌株處理組較對照組延遲2–4 d出現病徵，且罹病度分別僅有47.5%與30.2%，由此推測接種前1 d不論使用何種方式處理HTT-62均可延緩白菜軟腐病發病時間並降低罹病度。本研究證明B. amyloliquefaciens HTT-62為一具有防治白菜細菌性軟腐病的潛力菌株。 The bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) is one of the destructive diseases of Chinese cabbage in Taiwan. The purpose of this study was to screen and evaluate the potential antagonists for control of this disease. The six Bacillus spp. isolates, AC35, BAC15, NA0301, HTT-11, HTT-61, and HTT-62, showed superior antagonistic ability against Pcc strain Pcc10602. Thus, the effect on controlling this disease was evaluated on leaf tissue of Chinese cabbage by using puncture and spray method. The results showed that the disease severity of Pcc10602 inoculated-only were 100% at the 5th day after inoculation (DAI 5) by using two different methods. In addition, Bacillus amyloliquefaciens isolates BAC15, HTT-61, and HTT-62 significantly reduced the disease severity to 52.4%, 57.1%, and 57.1% at DAI 5 by using puncture method, respectively. In the greenhouse test, the B. amyloliquefaciens HTT-62 was applied to Chinese cabbage with using different methods (cotton-cover method and spray method). After 1 day, Pcc10602 was subsequently applied by the same method. The results revealed that the disease severity of the control treatment was 97.9% and 74.5% at DAI 12 by two different methods, respectively, while the disease severity of the treatment with HTT-62 was only 47.5% and 30.2%, respectively. Therefore, it is speculated that B. amyloliquefaciens HTT-62 can delay the occurrence of soft rot and reduce disease severity on cabbage in any way one day before inoculation. This study revealed that B. amyloliquefaciens HTT-62 is a potential antagonist to control the bacterial soft rot of Chinese cabbage.

Subjects

白菜

細菌性軟腐病

生物防治

芽孢桿菌

chinese cabbage

bacterial soft rot

biocontrol

bacillus

Publisher

農業試驗所

Type

journal article

File(s)

Name

72-1-1.pdf

Size

4.63 MB

Format

Adobe PDF

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