Osmotic Stress Promotes Shoot Regeneration in Immature Embryo-Derived Callus of Rice (Oryza sativa L.)
Other Title
滲透壓促進水稻未成熟胚誘導之癒合組織植株再生
Journal
中華農學會報
Journal Volume
3
Journal Issue
1
Pages
76-86
Date Issued
2002-02
Date
2002-02
Author(s)
Wen-Lii Huang
Yi-Chen Tsung
Li-Fei Liu
黃文理
曾一真
劉麗飛
Abstract
The callus regeneration ability of fifteen rice cultivars including 7 indica and 8 japonica type varieties under osmotic stress treatment were compared. The callus growth and cellular water status were further determined. The responses are different among rice cultivars as follows. Group l, shoots regenerated high frequency without any osmotic treatment. The frequency approximates to 75%. Group 2, shoot regeneration frequency, closed to 50-75%, was the highest by middle concentration of osmotic treatment. Group 3, shoot regeneration frequency, closed to 60-80%, was highest by highly osmotic treatment and only japonica type cultivars are included. Group 4, shoot regeneration is lower either with or without osmotic treatment. Further studies showed that the callus growth, water content, water potential and osmotic potential are decreased in shoot regenerable callus induced by osmotic
stress. These results suggest that the growth and differentiation of rice callus can be regulated by cellular water status itself.
本研究比較15個水稻品種(包括7個印度型與8個日本型品種)未成熟胚在滲透壓處理下誘導之癒合組織之植株再生率，並進一步測定滲透壓逆境下合組生長及細胞內水分狀態，以瞭解滲透壓處理對水稻癒合組織再生植株之影響。依其再生率差異，可將其區分為四大群；第一群不需經滲透壓處理即有高植怽再生率，其再生率約為75%左右；第二群僅需中度滲透壓處理即可獲得高株再生率，再生率介於50-75%之問，滲透壓處理濃度過高，再生率反而下降，第三群則需高濃度滲透壓處理才可獲得高值株再生率，其再生率介於60-80% 之間，且皆為日本型水稻品種；第四群不論有無滲透壓處理，植株再生率均低於25%。進一步的分析發現，在高滲透壓處理誘導之高植株再玍率合組織中，其鮮重、水分含量、水勢能、及滲透勢能均顯著下降，上述結果顯示影響水稻合組織生長與植株之再生可能與滲透壓藉由調節細胞內水分狀態有密切相關。
Subjects
cultivars
Oryza sativa
osmotic stress
shoot regeneration
栽培種
水稻
滲透逆境
植怽再生
Publisher
中華農學會
Type
journal article