本土化氣霧耕量產菌根接種源
Other Title
Mass Production of Mycorrhizal Fungus Inoculum by Domestic Aeroponic Culture
Journal
微生物肥料之開發與利用研討會專刊
Part Of
農業試驗所特刊第44號
Pages
165-172
ISBN
957-00-4053-X
Date Issued
1994-06
Author(s)
王均琍
Chun-Li Wang
Abstract
本研究以本省業者常用之氣霧耕設施，第一期以紅豆為寄主作物，於盆缽內播種之同時接種Glomus mosseae (Gm)，感染後以三種不同營養液作氣霧耕栽培，結果顯示於0.5強度Hoagland’s含l/4磷（H 1/4P）之營養液菌根孢子之產量優於0.5強度含全磷（1H 1P）及台肥液(200X)者。不同菌種產孢量亦有所不同，以Glomus etunicatum (26個孢子/1cm根段)與G. fasciculaturn高於G. mosseae(12個)。
第二期以蕃茄、玉米、百喜草為寄主作物，亦於盆缽內播種之同時接種G. etunicatum, C. fasciculatum與G. clarum四種菌之混合孢子土，感染後以氣霧耕栽培，結果顯示玉米植株生長至後期，植株高大不易固定，故後續試驗捨之。而百喜草每公分根段產孢量高於蕃茄，惟蕃茄菌根之生物量大於百喜草者，且蕃茄果實具經濟價值，值得繼續探討。
第三期仍以蕃茄、百喜草為寄主作物，以海棉塊育苗移作氣霧耕，之後以海棉塊內置菌根片段包覆蕃茄與百喜草根之周圍作菌根菌感染，惟海棉塊附近根系逐漸腐爛，乃將含菌根片段之細網（接菌網）覆於氣霧耕植株根之周圍，進行菌根菌接種，則生育期中寄主根系之發育完全不受影響，且菌根菌感染強度，囊孢與孢子產量均有增進。接菌八週後百喜草平均每公分根段孢子數30，蕃茄7.3。
就量產策略而言，本省蕃茄等經濟作物之栽培如能引用本研究所發展之方法，育苗期不予接菌，移植後，利用接菌網多次加強接菌，可大幅增加產孢量，業者之操作技術層面亦較簡便，適合大規模推廣應用。
Aeroponic culture by operating with domestic equipment was utilized to produce mycorrhizal fungus inoculum. In the first stage, adzuki bean seeded in pot was adopted as a host plant inoculatcd with Glomus mosseae (GM), After being precolonized, the seedlings of adzuki bean were transplanted in aeroponic culture. The results showed that among the three nutrient solutions used in this study, spore production of mycorrhizal fungus cultivated in 0.5 strength Hoagland’s solution containing 1/4 amount of phosphate was better than that in 0.5 strength Hoagland’s and Taifei nutrient solution (200X). Among the three mycorrhizal fungi used, spore productions of Glomus etunica turn (26 spores / I cm root section) and G. fasclculalum (24 spores) were more abundant than that of G. mosseae (12 spores).
In the second stage, tomato, corn and behia grass were used as host plants. The mixture of soil inoculnm of Glomus mosseae,G. etunicatum,G. fasciculatum and G. clarum were inoculated on host plants seedling in pots. After being precolonized by mycorrhizal fungi, seedlings were transplanted in aeroponie culture. The results showed the shoots of corn were too high to be handled with aeroponic culture, and was abandoned in later experiment. Although the spore production per 1 cm colonized root of bahia grass was more abundant than that in tomato. the biomass of colonized tomato roots was higher than that of bahia grass, and tomato has higher economic value, it is worthy to that tomato as a host crop to produce mycorrhizal fungus inoculum in further study.
In the third stage, tomato and bahia grass were used as host plants, seeded in spongy lump. After germination, seedlings were transplanted in aeroponic culture. The roots were packaged with a piece of sponge containig mycorrhizae fragments for inoculation. However, the roots near sponge were decaying gradually. Fine net containing mycorrhizae fragment (inoculation net) was adopted to wrap host roots cultivated in aeroponic culture to obtain inoculation, The results showed the development of host root is unaffected, and the infection strenght of mycorrhizal fungi, the production of vesicle and spore were improved. 8 weeks after inoculation, the spore number per 1 cm colonized root of bahia grase is 30, that of tomato is 7.3.
For mass production strategy in Taiwan, economic crop such as tomato, could highly improve its sporulation when seedlings were inoculated with mycorrhizal inoculation net after aeroponie culture. The inoculation technique developed in this study is convenient for producer, and therefor suitable for large application and extention.
Event(s)
微生物肥料之開發與利用研討會
Proceeding of A Symposium on development and Utilization of Microbial Fertilizer
Subjects
氣霧耕
內生菌根菌
量產
aeroponic culture
Mycorrhizal fungus
mass production
Publisher
臺灣省農業試驗所嘉義農業試驗分所
Type
conference paper
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