The production of imperatorin from angelica dahurica var Formosana by cell suspension culture
Other Title
利用細胞懸浮培養生產白芷中藥成份-Imperatorin的技術
Journal
中華農學會報
Journal Volume
168
Pages
32-48
Date Issued
1994-12
Date
1994-12
Author(s)
Tsay, H.S.
Chang, W.D.
Chen, C.C.
Chang, Y.S.
蔡新聲
張文德
陳忠川
張永勳
Abstract
Bai-Zhi (Angelica dahurica) is a valuable medicinal plant used as source of curatives for headache and skin disease in China. The purpose of this study was to establish cell suspension culture as alternative source of imperatorin, the major active ingredient of the species. The formulation of MS basic salts, modified by increasing the phosphate concentration to 2 mM and using an ammonium to nitrate ratio of 2:1, provided the best imperatorin production. Glucose was a better carbon source than sucrose and fructose. The addition of 0.5-1 mg/l of BA to the culture medium increased imperatorin yield, whereas auxins produced only negative effects. Supplementing the medium with 20g/l of the adsorbent Amberlite XAD-7 increased imperatorin yield 140-fold.
Subjects
Angelica dahurica
cell suspension culture
elicitors
imperatorin
medicinal compounds
secondary metabolites
白芷
細胞懸浮培養
誘引劑
中藥成份
二次代謝物
Publisher
中華農學會
Type
journal article