Evaluation of Reflectometry and Refractometry for Rapid Sugar Detection in Sweet Potatoes
Other Title
應用反射光度法及折射光度法於甘藷糖類含量快速檢測之可行性評估
Journal
台灣農業研究
Journal Volume
74
Journal Issue
2
Start Page
151
End Page
159
ISSN
2790-086X
Date Issued
2025-06-05
Author(s)
Abstract
Sugar content, a key quality attribute of sweet potatoes, is influenced by cultivar, maturi-ty, storage conditions, and processing methods. This study aimed to develop efficient methods for measuring sweet potato sugar content using the reflectometer and refractometer. Ten sweet potato cultivars with diverse tuber flesh colors were evaluated in raw and baked forms. A simplified water extraction method facilitated analysis with both instruments. The RQFlex20 reflectometer, coupled with Reflectoquant sucrose and total sugar (glucose and fructose) test kits, was used to measure the sugar concentrations in sweet potatoes. Results demonstrated two linear regression models between the reflectometer and the reference method for the corresponding sugar contents, with coefficients of determination (R2) of 0.95 and 0.86, respectively. Additionally, the refractometer measured total sugar content, exhibiting a strong linear regression (R2 = 0.91) with the reference method. Furthermore, it could predict maltose content in baked sweet potatoes with a linear regression model (R2 = 0.90), thereby overcoming a limitation of the RQFlex20 without maltose. This study demonstrated that re-flectometry and refractometry offered a rapid and cost-effective method for measuring sugar content in sweet potatoes. This provided a valuable alternative to traditional high-performance liquid chroma-tography (HPLC) techniques, particularly for the agricultural and food processing industries.
糖類含量為評估甘藷品質之重要指標，其受品種、成熟度、儲存條件及加工方式等因素而有不同的影響。本研究為開發可替代高效液相層析法 (high-performance liquid chromatography; HPLC) 之快速檢測方法，選用具有不同肉色的 10 種甘藷品種，利用簡化的水萃取法進行糖類萃取，並評估以反射式光度計與折射計檢測生鮮藷與烤藷糖類含量之可行性。試驗以反射式光度計 RQFlex20，搭配檢測試劑組進行分析。其糖類含量與參考方法 (HPLC) 之測值符合直線回歸模式，決定係數 (R2) 分別為 0.95 (蔗糖 ) 與 0.86 (葡萄糖與果糖)，顯示此方法具有可應用性。折射計與參考方法亦符合直線回歸模式，R2 為 0.91，能用以測定總糖 (蔗糖、葡萄糖、果糖及麥芽糖) 含量。另外，使用折射計能進一步預測烤藷中的麥芽糖含量 (R2 為 0.90)，可克服 RQFlex20缺乏專用麥芽糖檢測試劑組的限制。本研究應用反射式光度計與折射計，建立快速、經濟且高效檢測甘藷糖類含量的替代方法，有助於農民與加工業者在需要時更快地作出決策。
Subjects
Sweet potato
Sugar content
Reflectometer
Refractometer
甘藷
糖類含量
反射式光度計
折射計
Publisher
農業部農業試驗所
Type
journal article
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