茶葉中兒茶素類萃取及純化之研究
Other Title
Studies of the Extraction and Purification of Catechins in Tea
Journal
1997特用作物試驗成果研討會專刊
Part Of
農業試驗所特刊第74號
Pages
133-141
ISBN
957-02-1964-5
Date Issued
1997-08
Date
1997-08
Author(s)
陳英玲
阮逸明
Ying-Ling Chen
I-Ming Juan
Abstract
兒茶素類是茶葉中對人類具有生理活性的物質之一本試驗利用PVPP (Po1yvin-yloPlyPyrrolidone)吸附法,極性吸附法及親和性吸著等三種方法分別嘗試分離茶葉中的粗兒茶素類。結果顯示親和性吸著法能成功地分離出粗兒茶素類,尤其對酯型兒茶素類具有較高之專一性。萃取出之兒茶素類經脫鹽及冷凍乾燥後,可得粗兒茶素粉末。由於酯型兒茶素類具較高之抗氧化活性,故在抽取時宜選擇醋型兒茶素含量之茶樹品樹品種以得到較多之產物。
未來的研究方向為兒茶素類之大量抽取,及個別酯型兒茶素類之分離,期能擴大兒茶素類在食品加工上之利用。
Catechins in tea is one of the important compounds that have effective physiological activities on human bodies.Three methods were used to extract and purify catechins in tea liquor. The first method was made use of the absorption of polyvin-ylpolypyrrolidone(PVPP),the second the polar interations of aminopropyl group and the third covalent retention of phenyl-boronic acid group.It was shown that the third method nad best affinity formation occurred almost exclusively with diol,diamines or amino alcohol and successfully separated the caffeine and catechins in tea liquor.The powder of crude catechins could be obtained from those catechin extract which passed through the deaslt columns and freeze-drying.We should select the tea varieties to which have the higher contents of catechin gallate for extraction because the compound has higher antioxidant activities than that of simple forms.
To amplify the extractive quantity of catechins and to purify the individual catechins.especially catechin gallate,will be the future research priority.Expandig applications of catechins to the food industry is expected.
Event(s)
1997特用作物試驗成果研討會
Proceedings of A Symposium on the Experimental Results of Special Crops 1997
Subjects
茶葉
粗兒茶素
萃取
純化
Tea
Catechins
Extraction
Purification
Publisher
農業試驗所
Type
conference paper
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