以氣孔長度早期鑑定水稻花藥培養小株之倍數性

陳治官; 陳駿季; 黃真生; C.G. Chern; J.J. Chen; C.S. Huang; 陳治官;陳駿季;黃真生; C.G. Chern;J.J. Chen;C.S. Huang

doi:10.29951/JARC.198603.0004

以氣孔長度早期鑑定水稻花藥培養小株之倍數性

Other Title

Ploidy Identification of Rice Plantlets Derived from Anther Culture by Stomatal Size

Journal

中華農業研究

Journal Volume

35

Journal Issue

1

Pages

39-44

Date Issued

1986-03-04

Date

1986-03-04

Author(s)

陳治官

陳駿季

黃真生

C.G. Chern

J.J. Chen

C.S. Huang

DOI

10.29951/JARC.198603.0004

URI

https://scholars.tari.gov.tw/handle/123456789/1170

Abstract

為辨別花藥培養第一代（AC1S0）幼株的倍數性，以便早期行染色體倍加處理，取兩個水稻雜交組合之Fl，經花藥培養後所得AC1S0幼苗，以丙醯氛快速印模展開葉下表面，並觀察測量氣孔保衛細胞之長度。觀察氣孔的長度總共有16.86至31.36 μm之變域，平均長度為22.64 μm。然後以印模氣孔長度與AC1S0成株之倍數性相對照，發現單倍體株的氣孔長度有16.86至28.44 μm 之變域，平均為20.22 μm，雙倍體有17.67至31.36 μm之變域，平均為26.17 μm，兩者差異極顯著，證實以剛移出試管硬化後的AC1S0小株的氣孔保衛細胞長度，可以簡便而有效地辨出小株的倍數性，使單倍體小株可於早期倍加處理，以提高其在育種上的效率。 Stomatal size or guard cell length of the lower leaf surface of young rice plantlets derived from anther culture was investigated by the cyanoacrylate impression method. Plantlets from two japonica rice hybrids that received anther culture were used. The guard cell length of their AC1S0 plantlets as a whole ranged from 16.86 μm to 31.36 μm with a mean of 22.64 μm before the identification of ploidy. Highly significant difference in stomatal size was detected between haploid and diploid plantlets classified according to the morphological characteristics of adult plants in the paddy field. Stomatal size of young haploid plantlets varied from 16.86 μm to 28.44 μm with a mean of 20.18 μm and those of diploids from 17. 67 μm to 31. 36 μm with a mean of 26.17 μm. The results support the possibility of identifying the haploid plantlets from anther culture by this method and of doubling their chromosome number to obtain dihaploid for advancing rapidly the breeding cycle.

Publisher

農業試驗所

Type

journal article

File(s)

Name

journal_arc_35-1-4.pdf

Size

904.69 KB

Format

Adobe PDF

Checksum

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