應用RAPD技術對荔枝品種遺傳變異性之研究

Other Title
Using RAPD Markers to Study Genetic Variation among Lychee Cultivars
Journal
台灣農業研究
Journal Volume
56
Journal Issue
4
Pages
281-288
Date Issued
2007-12-03
Date
2007-12-03
Author(s)
鄧永興
林榮貴
Yung-Shing Teng
Rong-Quey Lin
DOI
URI
Abstract
以十三個荔枝品種爲材料，進行RAPD的片段分析。經篩選200個逢機引子後，選出23個引子所產生的259個條帶(RAPD markers)，做爲十三個荔枝品種間的親緣關係評估之依據。並利用Jaccard氏的計算方法，求得不同樣品間的相似度；並利用UPGMA方法，建立其親緣關係圖。 The random amplified polymorphic DNA (RAPD) markers were used to identify the genetic relationship among introduced lychee cultivars in this study. Among 200 different decamer primers, 23 primers were selected; the selected primers amplified a total of 259 DNA bands. The total number of amplified bands per primer varied from 9 (UBC-199) to 26 (OPG-08), on an average 11.3 bands were induced by each primer.
Subjects
遺傳變異
荔枝
逢機擴大多型性DNA
Genetic variation
Lychee
RAPD markers
Publisher
農業試驗所
Type
journal article
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