利用台灣金線連無菌播種根莖誘導多倍體研究
Other Title
In Vitro Induction of Polyploidy from Rhizomes of Anoectochilus formosanus
Resource
台灣農業研究 58(4): 302-309
Journal of Taiwan Agricultural Research 58(4): 302-309
Journal
台灣農業研究
Journal Volume
58
Journal Issue
4
Pages
302-309
Date Issued
2009-12-06
Date
2009-12-06
Author(s)
夏奇鈮
黃健覃
陳威臣
曹進義
梁淑惠
蔡新聲
Chi-Ni Hsia
Jian-Tan Huang
Uei-Chern Chen
Chin-Yi Tsao
Shu-Hui Liang
Shin-Sheng Tsay
Abstract
本研究以台灣金線連(Anoectochilus formosanus Hayata)無菌播種不同週齡之根莖(rhizome)作為培植體，並以流式細胞儀檢測新生芽體之倍體數，探討秋水仙素濃度、處理時間及固、液體處理方式對多倍體誘導之影響。比較固態或液態培養基添加0、0.025、0.125及0.625mM秋水仙素培養2週，對12週齡根莖培植體誘導多倍體之效果，結果顯示以0.625mM秋水仙素液態培養者，多倍體誘導率為44.8%最佳。
This study was conducted to investigate effects of concentration, exposure duration of colchicine and treating colchicine with liquid or solid agar medium on in vitro induction of polyploidy from rhizomes of Anoectochilus formosanus. Ploidy of shoots derived from rhizome explants were analyzed using a flow cytometry. When the 12-week-old rhizome explants were cultured for two weeks in a solid agar medium or in a liquid medium containing 0, 0.025, 0.125, and 0.625 mM colchicine, the induction rate of polyploidy was the highest (44.8%) for the regenerated shoots in the liquid culture containing 0.625mM colchicine.
Subjects
金線連
微體培養
秋水仙素
倍數體
流式細胞儀
Anoectochilus formosanus
Micropropagation
Colchicine
Polyploidy
Flow cytometry
Publisher
農業試驗所
Type
journal article
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