Crude Extracts of Agaricus brasiliensis Induce Apoptosis in Human Oral Cancer CAL 27 Cells through a Mitochondria-dependent Pathway

Fan, Ming-Jen; Lin, Yu-Chin; Shih, Hsin-Der; Yang, Jai-Sing; Liu, Kuo-Ching; Yang, Su-Tso; Lin, Chien-Yih; Wu, Rick Sai-Chuen; Yu, Chun-Shu; Ko, Yang-Ching; Chung, Jing-Gung

Crude Extracts of Agaricus brasiliensis Induce Apoptosis in Human Oral Cancer CAL 27 Cells through a Mitochondria-dependent Pathway

Journal

In Vivo

Journal Volume

25

Journal Issue

3

Pages

355-366

Date Issued

2011-06

Date

2011-06

Author(s)

Fan, Ming-Jen

Lin, Yu-Chin

Shih, Hsin-Der

Yang, Jai-Sing

Liu, Kuo-Ching

Yang, Su-Tso

Lin, Chien-Yih

Wu, Rick Sai-Chuen

Yu, Chun-Shu

Ko, Yang-Ching

Chung, Jing-Gung

URI

https://scholars.tari.gov.tw/handle/123456789/3959

https://iv.iiarjournals.org/content/25/3/355

Abstract

In Taiwan, oral cancer is the fourth leading cause of male cancer mortality, and is still increasing. The Basiodiomycete, Agaricus brasiliensis Mural (ABM) is a dietary mushroom and has been known for its immune-enhancing, antitumor, antioxidation, antiviral and anti-mutagenesis functions. However, the exact anticancer mechanisms of ABM on human oral cancer cells are still unclear. In the present study, we investigated the effects of 50% ethanol crude extracts and hot water extracts of ABM on oral cancer CAL 27 cells. We observed that 0.9 mg/ml and 0.7 mg/ml of ABM 50% ethanol crude extracts and hot water, respectively, caused morphological changes and significantly reduced cell viability after 48-h treatment. The results showed that both extracts of ABM inhibited cell proliferation, increased the Ca(2+) release, reduced the mitochondria membrane potential (Delta Psi m), and caused cell cycle arrest in the G(0)/G(1) I phase, which contributed to apoptosis. Additionally, ABM induced DNA fragmentation, a characteristic of apoptosis and the expressions of apoptosis-related proteins, including apoptosis-inducing factor, cytochrome c, and caspase-3, were increased. Overall, we demonstrated that 50% ethanol crude extract and hot water extracts of ABM were able to induce apoptotic cell death in CAL 27 cells via the release of cytochrome c from mitochondria into the cytoplasm and activation of caspase-3 in vitro.

Subjects

Agaricus brasiliensis

human oral cancer CAL 27 cells

apoptosis

mitochondria-dependent pathway

Publisher

International Institute of Anticancer Research

Type

journal article

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