Diploidization of Haploid Rice Plants by Colchicine Treatment
Other Title
秋水仙精處理單倍體水稻之倍加
Journal
中華農業研究
Journal Volume
28
Journal Issue
1
Start Page
45
End Page
49
Date Issued
1979-03-07
Date
1979-03-07
Author(s)
Ming-Hwa Lin
林明華
Abstract
Haploid plants derived from anther culture of three rice ( Oryza sativa L . ) varieties, Tainan 5, Taichung 65, aud Taichung Yu 196, were treated with four different concentrations ( 0.05, 0.1, 0.25, and 0.5%）of colchicine solution by leaf-sheath injection method . Diploid seeds were successfully obtained from the treated haploid plants. The percentage of plants producing diploid seeds and the percentage of tillers bearing diploid seeds was found to be 45 aod 3.2%, respectively. The number of diploid seeds per tiller ranged from 5.3 to 6.4 with an average of 5.7, while the number of diploid seeds per plant ranged from 9.3 to 19.2 with an average of 12. It is suggested that 0.05 or 0.1% colchicine solution is suitable for chromosome doubling in haploid rice plants by leaf-sheath injection method.
利用臺南5 號、臺中65 號及臺中育196號等三個水稻品種或品系之花藥培養誘導來的單借體植株，以四種不同濃度（0.05、0.1、0.25 和0.5%）的秋水仙精進行葉鞘注射法處理，可成功地獲得二倍體種子。單倍體植株產生二倍體種子及產生二倍體種子的分蘗的百分率分別為45%和3.2%。每分蘗的二倍體種子數在5.3 到6.4 粒之間，平均為5.7 粒，而每株的二倍體種子數在9.3 到19.2 粒之間，平均為12 粒。由試驗結果建議0.05 和0.1%的秋水仙精濃度可為葉鞘注射法處理單倍體水稻的染色體倍加之適宜濃度。
Publisher
農業試驗所
Type
journal article
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