Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels

Wen-Chi Hou; Yeh-Lin Lu; Sin-Yie Liu; Yaw-Huei Lin

Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels

Journal

Botanical Bulletin of Academia Sinica

Journal Volume

44

Journal Issue

1

Pages

37-41

Date Issued

2003-01

Date

2003-01

Author(s)

Wen-Chi Hou

Yeh-Lin Lu

Sin-Yie Liu

Yaw-Huei Lin

URI

https://scholars.tari.gov.tw/handle/123456789/1787

https://ejournal.sinica.edu.tw/bbas/toc/issue-2003-1.html

Abstract

Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65degreesC treatment; however, no SOD activity could be detected at either 70 or 80degreesC for 5 min. In contrast, all GPx isozymes were stable under 80degreesC for 5 min, and GPx in SL and TL cultivars could resist 80degreesC treatment for 30 min.

Subjects

Glutathione peroxidase

Liriope spicata L.

Superoxide dismutase

Publisher

Institute of Botany, Academia Sinica

Type

journal article