菜豆黃化嵌紋病毒及胡瓜嵌紋病毒在唐菖蒲葉片之分佈及其對病毒檢測之影響
Other Title
Variable distribution patterns of bean yellow mosaic potyvirus and cucumber mosaic cucumoviruses in gladiolus plant and their influence on virus detection.
Journal
植物病理學會刊
Journal Volume
8
Journal Issue
3
Pages
117-120
Date Issued
1999-09
Author(s)
Abstract
The concentrations of bean yellow mosaic potyvirus (BYMV) and cucumber mosaic cucumoviruses (CMV) accumulated in leaf tissues of different leaf orders of gladiolus plant were compared by ELISA in this study. The result showed that the distribution patterns of these two viruses in gladiolus plants were different. The former is evenly distributed in tissues throughout different leaf orders, although younger leaves tend to accumulate slightly higher concentrations than the older ones. On the contrary, CMV accumulates its concentration in the lower old leaves, therefore higher detection rates and EIA readings are always obtained from this leaf position. Furthermore, we have found that infection of CMV in some of the gladiolus varieties can only be detected by indexing the lowest old leaf but not the younger ones. This particular distribution pattern of CMV in gladiolus plants was further confirmed by inoculating the ELISA positive old leaf tissue on Chenopodium quinoa to develop local lesions, which were subsequently shown to react indistinguishably with standard CMV antigens in SDS- immunodiffusion test. Based on these results, it is proposed that indexing of BYMV and CMV infections in gladiolus plants should be conducted by sampling the lowest old leaf or mixing it with the youngest new leaf in order to obtain optimum results.
Subjects
gladiolus
BYMV
cmv
virus indexing
elisa
Publisher
中華民國植物病理學會
Type
journal article