由火球花所分離之Nerine latent virus 之血清學及分子生物學鑑定
Other Title
Serological and Molecular Identification of Nerine latent virus Infecting Blood Lily (Haemanthus multiflorus Martyn)
Journal
台灣農業研究
Journal Volume
65
Journal Issue
2
Pages
194-206
Date Issued
2016-06-09
Date
2016-06-09
Author(s)
Abstract
由未呈現病徵之火球花 (Haemanthus multiflorus Martyn) 植株葉片，經機械接種於奎藜 (Chenopodium quinoa Willd.) 葉片後出現局部斑點病徵，以3 次連續單一斑點分離獲得1 個分離株 (HM4)。將感染HM4 之火球花及其接種後之奎藜單斑組織於間接式- 酵素連結免疫分析法 (indirect enzyme-linked immunesorbent assay; indirect ELISA) 及西方墨點法中，均可與已知之Nerine latent virus (NeLV) 金花石蒜分離株 (NeLV-LY56) 之抗血清產生正反應。
A virus isolate (HM4), obtained from a symptomless blood lily (Haemanthus multiflorus Martyn) plant, was established on Chenopodium quinoa Willd. by three successive single lesion reisolation and inoculation.
Subjects
火球花病毒病
納麗石蒜潛隱病毒
血清檢測
RT-PCR 檢測
類緣分析
Blood lily (Haemanthus multiflorus Martyn)
Nerine latent virus (NeLV)
Serological detection
RT-PCR detection
Phylogenetic analysis
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
65-2-9.pdf
Size
2.42 MB
Format
Adobe PDF
Checksum
(MD5):9bce3167eab304ebee495670e2d963ca