熱療法培育本省無病毒優良柑橘種苗
Other Title
Heat Therapy for the Production of Virus-Free Citrus Plants in Taiwan
Start Page
103
End Page
111
Date Issued
1989-12
Date
1989-12
Author(s)
黃秋雄
徐信次
Huang Chiou-hsiung
Hsu Hsin-tszu
Abstract
本文主要討論熱療對本省柑橘三種系統性病害，即柑橘立枯病（黃龍病）、南非立枯病(tristeza virus)及鱗砧病(exocortis)之抑制效果。另從優良母樹選找經熱療處理、苗木繁殖及指示植物檢定來培育無病毒種苗之經過與其間發現的問題也加以說明。綜合各項試驗所得結果，立枯病病原易於經由熱療處理而使之不活化，tristeza病毒雖然對熱較具耐性，但可經由新生芽條取得無病苗，鱗砧病似乎很難用熱療法去除之。自全省各主要柑橘產區所選出之優良母樹，經園藝特性分析，採穗嫁接後熱療，再繁殖幼苗經指示植物檢定，共選出椪柑及桶柑優良無病毒原種苗各11棵，目前部份尚保存於農試所。然而以相同方法施於柳橙及晚崙夏橙未獲成功，其因乃所有複選合格甜橙母樹均潛伏感染鱗砧病，熱療法難以將本病去除之。未來本省對於含有鱗砧病或其他不易以熱療去除之病毒的柑橘品種，勢必以熱療配合shoot-tip grafting或經由珠心胚系選拔來取得無病毒種苗。
This paper deals with the effect of heat treatment on likubin pathogen, tristeza virus and exocortis viroid, and the use of heat therapy for the production of virus-free citrus plants in Taiwan. Based on the reports, likubin diseased buds grafted on Rangpur lime seedlings exposed to the alternative temperatures of daytime 40℃ for l6hr and night time 30℃ for 8hr for 4 weeks or longer, new growth from the diseased buds showed no likubin symptoms, indicating that likubin pathogen was inactivated. However, tristeza virus was not inactivated by the same treatment. When young shoots from tristeza infected mexican lime seedlings which were placed at alternative temperatures of 40/30℃ for 15 weeks or 39-45/29-33℃ for 10 weeks could be free from tristeza virus. Alternative temperatures of 38/28℃ seemed not sufficient for the inactivation of tristeza. Because none of the heat-treated cuttings were free from exocortis even the treatment was prolonged up to 52 weeks, heat treatment at the above conditions seemed difficult to inactivate the exocortis. Application of heat therapy for the production of virus-free plants has been succeeded in some citrus cultivars. According to the field selection of parent source trees, analysis of horticultural characters, and indexing results, virus-free citrus plants for Ponkan and Tankan cultivars were obtained. However, due to the latent infection of exocortis in Liuchen and Valencia orange, no virus-free plants were obtained in these two cultivars.
Event(s)
柑橘試驗研究成果專題研討會
Proceeding of a Symposium on the Researches and Development of Citrus in Taiwan
Subjects
熱療
無病毒苗
立枯病
南非立枯病
鱗砧病
heat therapy
virus-free plants
likubin
tristeza
exocrtis
Publisher
臺灣省農業試驗所
Type
book part
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publication_no27-18.pdf
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1.16 MB
Format
Adobe PDF
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