培養基組成分對高氏柴胡癒合組織增殖與柴胡皂苷含量之影響

陳威臣; 夏奇鈮; 葉茂生; 曹進義; 蔡新聲; Uei-Chern Chen; Chi-Ni Hsia; Mau-Shing Yeh; Chin-Yi Tsao; Hsin-Sheng Tsay; 陳威臣;夏奇鈮;葉茂生;曹進義;蔡新聲

doi:10.6156/JTAR/2008.05702.04

培養基組成分對高氏柴胡癒合組織增殖與柴胡皂苷含量之影響

Other Title

Influence of Medium Components on Callus Proliferation and Saikosaponin Content of Bupleurum kaoi Liu, Chao et Chuang

Resource

台灣農業研究 57(2): 119-132

Journal of Taiwan Agricultural Research 57(2): 119-132

Journal

台灣農業研究

Journal Volume

57

Journal Issue

2

Pages

119-132

Date Issued

2008-06-04

Date

2008-06-04

Author(s)

陳威臣

夏奇鈮

葉茂生

曹進義

蔡新聲

Uei-Chern Chen

Chi-Ni Hsia

Mau-Shing Yeh

Chin-Yi Tsao

Hsin-Sheng Tsay

DOI

10.6156/JTAR/2008.05702.04

URI

https://scholars.tari.gov.tw/handle/123456789/12085

Abstract

本研究利用高氏柴胡(Bupleurum kaoi Liu, Chao et Chuang)葉片癒合組織培養於添加0.2 mg/L 2,4-二氯苯氧乙酸(2,4-dichlorophenoxyacetic acid; 2,4-D)之1/2 MS(Murashige & Skoog 1962)基本培養基(D2培養基)，探討其他培養基組成分的添加對癒合組織增殖與柴胡皂苷(saikosaponins)含量之影響。 Medium components and their best concentrations of cytokinin, sucrose, Difco Bacto-agar, peptone, casein hydrolysate, coconut water were separately tested for callus growth of Bupleurum kaoi Liu, Chao et Chuang. Saikosaponins production of callus tissues was monitored.

Subjects

柴胡

藥用植物

細胞培養

二次代謝物

高壓液相層析

柴胡

藥用植物

細胞培養

二次代謝物

高壓液相層析

Bupleuri Radix

Medicinal plant

Cell culture

Secondary metabolite

High performance liquid chromatography

Bupleuri Radix

Medicinal plant

Cell culture

Secondary metabolite

High performance liquid chromatography

Publisher

農業試驗所

Type

journal article

File(s)

Name

journal_arc_57-2-4.pdf

Size

1.18 MB

Format

Adobe PDF

Checksum

(MD5):d91b0a99df47bc99ce72f8fdf9f4033e