Extraction of RNA from tissues containing high levels of procyanidins that bind RNA

Chang-Sheng Wang; Lila O. Vodkin

doi:10.1007/BF02668374

Extraction of RNA from tissues containing high levels of procyanidins that bind RNA

Journal

Plant Molecular Biology Reporter

Journal Volume

12

Journal Issue

2

Pages

132-145

Date Issued

1994-06

Author(s)

Chang-Sheng Wang

Lila O. Vodkin

DOI

10.1007/BF02668374

URI

https://link.springer.com/article/10.1007/BF02668374

https://scholars.tari.gov.tw/handle/123456789/21153

Abstract

A modified phenol-LiCl method for the extraction of RNA from seed coats of genotypes L62-1058 and L66-14 (yellow and black seed coated, respectively) of near isogenic lines of soyabean Clark that yielded high quality RNA is described. Seed coat material was ground in a buffer containing a high concentration of bovine serum albumin (100 mg BSA/50 mg of lyophilized seed coats) to competitively inhibit proanthocyanidin binding. The presence of hydrated insoluble polyvinylpolypyrrolidone (PVPP) was also necessary to bind proanthocyanidins and remove them from solution.

Subjects

soybean

glycine max

procyanidins

flavonoids

Publisher

Springer

Type

journal article