Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp niveum

Ying-Hong Lin; Kan-Shu Chen; Tsung-Dao Liou; Jenn-Wen Huang; Pi-Fang Linda Chang

Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp niveum

Journal

Botanical Studies

Journal Volume

50

Journal Issue

3

Pages

273-280

Date Issued

2009-07

Author(s)

Ying-Hong Lin

Kan-Shu Chen

Tsung-Dao Liou

Jenn-Wen Huang

Pi-Fang Linda Chang

URI

https://ejournal.sinica.edu.tw/bbas/toc/issue-2009-3.html

https://scholars.tari.gov.tw/handle/123456789/16150

Abstract

The vascular wilt disease of watermelon caused by Fusarium oxysporum f. sp. niveum (Fon) is one of the major factors limiting watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) production worldwide. Breeding highly resistant lines is one of the best ways to control the disease. In order to accelerate the breeding programs, a reliable PCR (polymerase chain reaction) technique was developed to rapidly differentiate the disease-resistant watermelons. The G05-SCAR (sequence characterized amplified region) primer set GsF5/GsR5, derived from the OP-G05 random primer-amplified fragment sequence, produced an 898 bp DNA fragment which was specific to watermelon lines resistant or tolerant to Fon. With optimized PCR assay, the Molecular method could easily differentiate Fon-resistant and -susceptible hybrid watermelon populations.

Subjects

Breeding programs

citrullus lanatus

fusarium oxysporum f. sp. niveum

fusarium wilt

molecular detection

molecular marker

pcr

RAPD

SCAR

Publisher

Academia Sinica

Type

journal article