植物生長調節劑與兩階段培養對薑組織培養苗增殖與生長之影響
Other Title
Effect of Plant Growth Regulator and Two-Stage Culture on Proliferation and Growth of In Vitro Ginger Plantlets
Resource
台灣農業研究 67(4):401-413
Journal of Taiwan Agricultural Research 67(4):401-413
Journal
台灣農業研究
Journal Volume
67
Journal Issue
4
Pages
401-413
Date Issued
2018-12-08
Date
2018-12-08
Author(s)
陳威臣
曹進義
吳姿穎
夏奇鈮
Uei-Chern Chen
Chin-Yi Tsao
Tzu-Ying Wu
Chi-Ni Hsia
Abstract
本研究利用「廣東薑」（Zingiber officinale ‘Guang Dong’）與「竹薑」（Z. officinale ‘Chu’）根莖之新生芽體為培植體，建立其組織培養苗大量繁殖方式。切取長約1 cm之新生根莖芽體（rhizome bud）為培植體，先利用75%酒精處理30 s後，再以0.6% NaOCl消毒15 min，培養於含有0.4 mg L^(-1)苯甲基腺嘌呤（6-benzylaminopurine;BA）之B5固態培養基中12 wk後，建立無菌培養瓶苗。
Efficient protocols for in vitro multiplication of Zingiber officinale 'Guang Dong' and 'Chu' were established in this study. Newly germinated rhizome buds (about 1 cm) were treated with 75% alcohol for 30 s, followed by 0.6% NaOCl for 15 min, before cultured on a B5 basal medium containing 0.4 mg L^(-1) 6-benzyladeninepurine (BA) for 12 wk to establish in vitro culture.
Subjects
薑
液-固態培養
苯甲基腺嘌呤
大量繁殖
Zingiber officinale
Liquid-solid culture
6-benzylaminopurine
Mass propagation
Publisher
農業試驗所
Type
journal article
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