玉米褪綠斑駁病毒 (Maize chlorotic mottle virus) 之親緣分析與台灣之發生調查

Other Title
Phylogenetic Analysis and Occurrence of Maize chlorotic mottle virus Infecting Maize in Taiwan
Resource
台灣農業研究 67(2): 191-208
Journal of Taiwan Agricultural Research 67(2): 191-208
Journal
台灣農業研究
Journal Volume
67
Journal Issue
2
Pages
191-208
Date Issued
2018-06-05
Date
2018-06-05
Author(s)
周建銘
陳君弢
簡伊萱
蔡錦慧
陳怡如 
鄧汀欽 
Chien-Ming Chou
Chun-Tao Chen
Yi-Hsuan Chien
Chin-Hui Tsai
Yi-Ju Chen 
Ting-Ching Deng
DOI
URI
Abstract
自雲林縣虎尾地區採集具褪綠、黃化及褐化乾枯病徵之玉米病害樣本，並將其機械接種於甜玉米'Honey-236'可產生類似褪綠斑駁病徵，並獲得一個分離株(稱為NFU2)。樣本以簡併式引子對進行反轉錄聚合酶鏈鎖反應(reverse transcriptase polymerase chain reaction; RT-PCR)，可增幅出預期大小(1.4 kb)產物，經解序後序列與玉米褪綠斑駁病毒(Maize chlorotic mottle virus; MCMV)中國Yunnan3分離株(Acc. No. JQ982469)相同度達99%。 Maize samples showing chlorotic, yellowing and leaf necrosis symptoms were collected in Huwei Township, Yunlin County in February 2014. After inoculation by leaf sap, the similar chlorotic mottle symptom was developed on sweet corn cv. 'Honey-236' and a pure virus isolate ＂NFU2＂ was obtained from infected sweet corn by a serial inoculation. The total RNA of ＂NFU2＂ isolate was extracted to conduct a reverse transcriptase polymerase chain reaction (RT-PCR) with the degenerate primer (Tenui-DF1/Tenui-DR1) and that yields a 1.4 kb product. The product could combine into one single contig, and this contig shared 99% sequence identities with Chinese Maize chlorotic mottle virus (MCMV) ＂Yunnan3＂ (Acc. No. JQ982469).
Subjects
玉米褪綠斑駁病毒
甘蔗嵌紋病毒
親緣分析
檢測試劑
病害調查
Maize chlorotic mottle virus
Sugarcane mosaic virus
Phylogenetic analysis
Detection reagent
Field survey
Publisher
農業試驗所
Type
journal article
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