Effects of Nitrogen Supply to Donor Plants on Pollen Embryogenesis in Cultured Tobacco Anthers
Other Title
不同氮素量生長下菸草對花葯培養胚狀體形成的影響
Journal
中華農業研究
Journal Volume
30
Journal Issue
1
Pages
5-13
Date Issued
1981-03-02
Date
1981-03-02
Author(s)
Hsin-Sheng Tsay
蔡新聲
Abstract
Levels of nitrogen supplied to culture solution had definite effects on the development and physiological aspects of tobacco plants (Nicotiana tabacum L. cv. Wisconsin 38). The correlation between nutritional status of the source plants and embryogenic characteristics of the excised anthers cultured in vitro, as well as the related histological explanations were demonstrated. Based on Hoagland’s basic salts, 15 mM of nitrogen supplied to culture solution was most optimal for plant growth among the five nitrogen levels (0, 1.5, 4.5, 15.0, and 45.0 mM ) tested. The highest embryogenic ability of cultured anthers was obtained when donor plants were subjected to 15 mM nitrogen treatment. Low nitrogen supply lowered the embryogenic ability. Histological study on microsporogenesis in vivo revealed that anhers obtained from the O nitrogen treatment had poorly developed tapetum layer ad contained microspore with lightly stained cytoplasm. It was proposed that a better-developed tapetum layer and microspores with densely stained cytoplasm ware important for haploid embryogenesis in tabacco anther culture.
Publisher
農業試驗所
Type
journal article
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