水稻 T-DNA 突變種原庫之建立與營運
Other Title
The Establishment and Operation of a Rice T-DNA Mutant Genebank
Journal
中華農學會報
Journal Volume
5
Journal Issue
6
Pages
514-525
Date Issued
2004-12
Abstract
基因功能消除或基因功能活化法是研究基因功能最直接的路徑，以 T-DNA 插入基因產生突變，為最普遍被用來消除或活化基因功能的方法。為了評估需要多少個品系才能使每個基因皆可為 T-DNA 插入，必須考慮三個決定 T-DNA 插入機率的因子：基因的大小、基因組的大小及 T-DNA 插入的數目，以水稻而言預估大約需要 246,000 個 T-DNA 插入品系才能使基因組達到飽和突變。
The technologies of gene knockout and activation tagging provide a direct route to determining the function of a gene. T-DNA insertion mutagenesis is one of the most popular methods for understanding gene function. Three variables, namely the size of the gene, the size of the genome and the number of T-DNA inserts, were used to determine the probability that a T-DNA insert will be found within a given gene distributed amongst the population.
Subjects
水稻
基因功能消除法
t-dna
rice
transfer dna
gene knockout
Publisher
中華農學會
Type
journal article