Insecticide Resistance Study in Plutella xylostella (L.)－IV. The Activities of Glutathione-S-transferase in the Organophosphorus-resistant Strains
Other Title
小菜蛾抗藥性之研究－IV. Glutathione-S-transferase在抗有機磷品系中之變化
Journal
中華農業研究
Journal Volume
32
Journal Issue
4
Pages
373-378
Date Issued
1983-12-09
Date
1983-12-09
Author(s)
Edward Y. Cheng
Tao-Mei Chou
Ching-Hua Kao
鄭允
周桃美
高靜華
Abstract
The role of glutathione(GSH)-S-transferase as a detoxication mechanism to organophosphorus insecticides was investigated in the diamondback moth, Plu/ella xylostella (L.). The GSH-S-transferase was prepared fromthe susceptible IL-strain, two organophosphorus-resistant strains and two synthetic pyrethroid-resistant strains. The result indicated that the GSH-S-transferase activities in two organophosphorus- resistant strains were three to four times higher than that of the susceptible IL-strain, i.e., the parental strain from which all resistant strains were derived. Both O-methyl and O-ethyl groups on the organophosphorus insecticides can cause GSH-S-transferase activity increases in insecticide pressed diamondback moth population. Hence, the GSH-S-transferase detoxication mechanism is quite specific to the organophosphorus insecticides. The study indicated that the glutathione conjugation is an important resistant mechanism to the organophosphorus insecticides in the diamondback moth.
利用在宜蘭所採得之感性品系小菜蛾，在室內誘發抗佈飛松及抗美文松兩有機磷劑品系，再行比較該等品系小菜蛾體內解毒酵素GSH-S-transferase活性之變化。試驗中利用抗百滅靈及抗賽滅寧二品系作對照，結果發現該解毒酵素的確在抗有機磷品系中提高約四倍，幾乎相當於該品系實際增加之抗性指數（ Resistant Ratio ，而抗合成除蟲菊精之品系均無此酵素活性提高之現象，顯然GSH-S-transferase係一抗有機磷之專有機制，此結果與國外報導GSH-S-transferase為家蠅抗有機磷之主要機制結果相符，因此證明此一抗藥機制亦存在於小菜蛾體內。
試驗中並發現，小菜蛾體內之GSH-S-transferase經分離出體外，活性就開始下降，添加 EDTA 及 BSA 均不能阻止活性下降之現象及程度，三小時內活性下降接近零點。
Publisher
農業試驗所
Type
journal article
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