Nature of Resistance to Methyl Benzimidazole Carbamate Fungicides in Fusarium oxysporum f.sp. lilii and F. oxysporum f.sp. gladioli in Taiwan

Wen Hsin Chung; Wen Chuan Chung; Pen Fen Ting; Chang Chien Ru; Hung Chang Huang; Jenn Wen Huang

doi:10.1111/j.1439-0434.2009.01545.x

Nature of Resistance to Methyl Benzimidazole Carbamate Fungicides in Fusarium oxysporum f.sp. lilii and F. oxysporum f.sp. gladioli in Taiwan

Journal

Journal of Phytopathology

Journal Volume

157

Journal Issue

11-12

Pages

742-747

Date Issued

2009-12

Date

2009-12

Author(s)

Wen Hsin Chung

Wen Chuan Chung

Pen Fen Ting

Chang Chien Ru

Hung Chang Huang

Jenn Wen Huang

DOI

10.1111/j.1439-0434.2009.01545.x

URI

https://scholars.tari.gov.tw/handle/123456789/14316

https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01545.x

Abstract

Fusarium oxysporum f.sp. gladioli and F. oxysporum f.sp. lilii cause corm rot and yellowing of gladiolus and lilies respectively. Resistance among isolates of these two pathogens to two benzimidazole (methyl benzimidazole carbamate: MBC) fungicides, benomyl and thiabendazole, has been identified. However, sensitivity to other benzimidazole fungicides among isolates of F. oxysporum f.sp. gladioli and F. oxysporum f.sp. lilii remains unclear. Sensitivity among isolates of these two pathogens to benomyl, thiophanate-methyl, carbendazim and thiabendazole were evaluated in this study. Results showed that among six F. oxysporum f.sp. gladioli isolates, one was highly resistant, four were moderately resistant and one was highly sensitive to benomyl, five were highly resistant to thiophanate-methyl, four were sensitive and one was highly sensitive to carbendazim and all six isolates were sensitive to thiabendazole. Among the 13 F. oxysporum f.sp. lilii isolates tested, three were moderately resistant and 10 were highly sensitive to benomyl, and five were highly resistant, three were moderately resistant and five were sensitive to thiophanate-methyl. While all isolates were highly sensitive or sensitive to carbendazim and thiabendazole. These results strongly suggest that the mode of fungicide resistance in these two pathogens varies among members of the benzimidazole fungicide group and that cross resistance does not always occur. Sequence analysis of the partially amplified β-tubulin gene indicated that mutation in codons 198 and 200 had not occurred in the benzimidazole resistant isolates. It was concluded that resistance found in Taiwan isolates of F. oxysporum f.sp. gladioli and F. oxysporum f.sp. lilii to these fungicides may be due to some other mechanism.

Subjects

Fusarium oxysporum f.sp. gladioli

F. oxysporum f.sp. lilii

β-tubulin

benzimidazole

fungicide resistance

Publisher

Wiley

Type

journal article

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