Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus

Kuo-Shiou Huang; Chia-Hsing Tai; Ying-Huey Cheng; Shu-Hui Lin; Tsung-Chi Chen; Fuh-Jyh Jan

doi:10.1007/s00705-017-3260-1

Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus

Journal

Archives of Virology

Journal Volume

162

Journal Issue

7

Pages

2109-2113

Date Issued

2017-07

Date

2017-07

Author(s)

Kuo-Shiou Huang

Chia-Hsing Tai

Ying-Huey Cheng

Shu-Hui Lin

Tsung-Chi Chen

Fuh-Jyh Jan

DOI

10.1007/s00705-017-3260-1

URI

https://scholars.tari.gov.tw/handle/123456789/4166

https://link.springer.com/article/10.1007%2Fs00705-017-3260-1

Abstract

Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.

Publisher

Springer-Verlag

Type

journal article

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